高效液相色谱法测定补气养血汤中梓醇含量  

Determination of Catalpol in the decoction for replenishing Qi and nourishing blood by high performance liquid chromatography

作　　者：李艳翠[1] 刘娟[2] 牛效清[1] 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154003 [2]佳木斯大学化学与药学院,黑龙江佳木斯154007

出　　处：《黑龙江医药科学》2007年第5期23-24,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的:建立补气养血汤中梓醇的含量测定方法。方法:采用HPLC法,以乙腈—水(0.5∶99.5)为流动相,210nm为检测波长,对补气养血汤中梓醇含量进行了测定。结果:此方法的平均回收率为99.9%,RSD为1.79%,补气养血汤中梓醇的含量不少于0.3152m g/mL,其保留时间为18.50min。结论:本方法操作简便,快捷,准确,可用于该制剂的初步质量控制。Objective. To establish the determination standards of Catalpol in the decoction for replenishing Qi and nourishing blood. Methods ：We used high performance liquid chromatography at 210nm with acetonitrile-water （0.5：99.5） as a mobilephase to determine the content of Catalpol. Results：The average recovery was 99. 9% and RSD was 1. 79%, and the content of Catalpol in the decoction for replenishing Qi and nourishing blood was no less than 0. 3152mg/mL , the retention time was 18. 50 minutes. Conclusion：The methods is simple, quick, accurate and can be used for the prelimary quality control of the decoction for replenishing Qi and nourishing blood.

关 键 词：HPLC 梓醇 补气养血汤 

分 类 号：R927.2[医药卫生—药学]