眼镜蛇毒组分C对小鼠实验性肝癌细胞生长影响的探讨  

Study of Effects of Fraction C from Chinese Cobra on Growth of Experimental Liver Cancer Cells in Mice

作　　者：林振桃[1] 赵路宁[1] 植飞 管锦霞[1] 罗肖萍[1] 

机构地区：[1]广州医学院广州蛇毒研究所

出　　处：《蛇志》1997年第1期3-6,共4页Journal of Snake

基　　金：广东省科委基金

摘　　要：ＢＡＬＢ／Ｃ小鼠腹水型肝癌细胞与眼镜蛇毒组分Ｃ在无菌条件下混合后接种于昆明种小鼠右前肢腋下，在２５℃条件下饲养１２天。结果表明，组分Ｃ能明显抑制小鼠肝癌细胞的生长（ＩＣ５０为５９．０８μｇ／ｍｌ），当肝癌细胞膜被人为造成损伤时，组分Ｃ对其生长的抑制作用则显著增强（ＩＣ５０为６．７２μｇ／ｍｌ）。此外，组分Ｃ能显著延长荷瘤小鼠的生存时间。对体外培养的小鼠肝癌细胞，组分Ｃ也具有很强的杀伤作用，且这种杀伤作用受组分Ｃ的浓度、组分Ｃ与肝癌细胞孵育的时间及孵育的温度的影响。病理组织检查发现，给组分Ｃ后，镜下可见肝癌细胞生长受到明显抑制，肝癌细胞未向皮肤及附件浸润。BALA/C mice bearing ascitic liver cancer cells(5×10 7/ml) was mixed with diffrent dose of Fraction C from Chinese Cobra together in no-bacteria condition This mixed substanece was retransplated to mice,and then these mice were killed after 12 days,the experimental results showed growth of the cancer cells was inhibited significantly(IC 50 was59 08μg/ml) If the cancer cells membrane had injured before mixing with Fraction C,this inhibition was increase markedly (IC 50 was 6 72μg/ml) Forthermore,Fraction C could inflict liver cancer cells which was cultured outside body and this inflicts action in canncer cells far more than that in red blood cells. Retransplanted experimental results also indicated the survival period of mice which was gived Fraction C was prolonged significantly After giving Fraction C 12 days,the pathological tissue check showed the cancer cells growth was inhibited The cells didn’t intrude into the animal skin There were a few areas of necroses in group of cancer cells All the results suggested Fraction C could inhibit growth of liver cancer cells

关 键 词：眼镜蛇毒组分C 肝癌 癌细胞 抑制作用 药理学 

分 类 号：R735.705[医药卫生—肿瘤] R996.3[医药卫生—临床医学]