三个山羊品种KAP8基因的PCR-SSCP分析  

Analysis of Polymorphism on KAP8 Gene in Three Goat Breeds with PCR-SSCP

作　　者：赵俊星[1] 任有蛇[1] 岳文斌[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《生物技术》2007年第5期3-6,共4页Biotechnology

基　　金：山西省重大科技发展计划招标项目(031041);山西省科技攻关项目(031044)资助

摘　　要：目的:为了筛选绒山羊产绒性状的候选基因。方法:采用PCR-SSCP法对三个山羊品种角蛋白辅助蛋白8(KAP8)进行了多态性研究。结果:三个品种中KAP8.1均未出现多态,而KAP8.2均出现了三种带型:AA、BB和AB。Hardy-Weinberg分析表明辽宁绒山羊和黎城大青羊未达到平衡状态,辽-苛高代杂种山羊达到了平衡。测序结果显示,和Genebank登陆序列相比,AA和BB均发生了碱基变化。其中AA型出现了以下变异:216bp(G-A),217(A-G),BB型出现了以下变异:216bp(G-A),232bp(C-T)。分析表明AA型变异造成了氨基酸序列发生了变化(R-K),而BB型突变未造成氨基酸发生改变。结论:KAP8.2可能是影响绒山羊产绒性状的基因之一。Selecting the candidate gene for cashmere. Methods： The polymerase chain reaction- single strand conformation polymorphism （PCR- SSCP）was used to detected the polymorphism of keratin associated protein 8 （KAPS） in three goat breeds. Results： The results show that the KAPS. 1 did not present polymorphism in all three breeds, and the KAPS.2 shows three Genotypes： AA, AB and BB. The frequency of all pattern types analyzed by PCR- SSCP were Hardy - Weinberg equilibrium in Liaoning goat breed and Licheng goat breeds, however the same result didn＇t appear in Liaoning - Kelan crossed goat breed. The The results indicated that both AA and BB have nucleofide substitution , different genotype homozygote on KAP8.2 were cloned and sequenced. the AA genotype presents the following mutation： 216bp（G - A） ,217（A - G） ,and the BB genotype： 216bp（G- A）,232bp（C- T）o The sequence of amino acids was analyzed, the results show that the mutations in AA genotype caused an amino acid change（R- K）, but BB genotype did not cause the same results. Conclusion： All the analysis above indicated that the KAPS.2 gene may be one of the candidate genes that influence the cashmere traits.

关 键 词：辽宁绒山羊 黎城大青羊 KAP8 PCR-SSCP 

分 类 号：S828[农业科学—畜牧学]