低温下皮肤力学信号相关蛋白表达与活力关系研究  

Study on relationship between expression of mechanical signal related proteins and validity of skin after cryopreservation

作　　者：贾晓明[1] 马彩虹[1] 于瑜[1] 姚咏明[1] 董宁[1] 

机构地区：[1]解放军总医院第304临床部全军烧伤研究所,北京100037

出　　处：《军医进修学院学报》2007年第5期356-357,共2页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目(30070298;30672177);首都医学发展科研基金项目(2005-3087)

摘　　要：目的:探讨皮肤力学信号相关蛋白整合素(integrin)、踝蛋白(talin)在不同低温条件下的表达与皮肤活力改变的关系,进一步揭示皮肤低温损伤的机制。方法:从同一个体取标本后用5种不同的温度保存,分为新鲜组、4℃组、-20℃组、-80℃组和-196℃组,并在保存48 h后进行实验观察,用integrinβ1、talin抗体对5种储存条件下的皮肤作免疫组化染色,图像分析方法定量,同时采用氧耗量的方法对不同低温保存后皮肤活力进行检测。结果:新鲜组、-196℃组、-80℃组、-20℃组、4℃组活力依次下降,同时-80℃组、-20℃组、4℃组两种蛋白表达亦有明显下降。结论:低温保存后活力的改变与皮肤力学信号相关蛋白的表达下降关系密切。Objective：To investigate the changes of integrin β1 and talin in epidermal cells treated by different temperatures. Methods：The independent experiments were performed with samples from the same individual. These samples were preserved by 4 methods： 4℃, - 20 ℃, - 80℃, - 196℃, to be compared with fresh skin. The experiments were carried out 48 hours after preservation, Immunohistochemistry technology was adopted for the examination. Then the histological structure of skin of different groups was observed and analyzed by pathological technology（ immunohistochemical staining）. Furthermore, we investigated the viability of skin in five respective groups evaluated using oxygen consumption assay. Results：integrin β1 and talin were affected in different temperature for preservation. In response to lowering of temperature, the content of integrin β1 and talin were increased. The mechanism underlying these activities might involve damage of skin by low temperature and various alterations appeared while the samples were stored at different temperatures. The skin which was preserved at - 196 ℃ showed less effect compared with other groups. Conclusion：These results demonstrate that the cryoinjury of skin is correlated with changes of the two proteins, and integrin β1 and talin play important roles in cryopreservation.

关 键 词：皮肤 低温损伤 力学信号 整合素Β1 踝蛋白 

分 类 号：R329.499[医药卫生—人体解剖和组织胚胎学]