基于聚苯胺掺杂乙醇胺固定DNA电化学传感器的研究  

Study on DNA Electrochemical Sensor Based on Ethanolamine Doped Polyanline Immobilized DNA

作　　者：时伟杰[1] 艾仕云[1] 张玉玮[1] 朱鲁生[2] 

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271018 [2]山东农业大学资源与环境学院,山东泰安271018

出　　处：《分析科学学报》2007年第5期507-510,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.20477022);山东省自然科学基金(No.Y2006B20)

摘　　要：本研究以电化学聚合法制备了聚苯胺掺杂乙醇胺修饰电极,并成功固定了DNA探针。文中对修饰电极的制备和DNA的固定杂交条件进行了探讨,并利用循环伏安法测定嵌入双链DNA(dsDNA)分子碱基对中的亚甲基蓝的氧化还原峰电流,识别和测定溶液中互补的单链DNA(ssDNA)片段,从而实现对溶液中不同基因片段的检测。In this paper, ethanolamine doped polyamine modified electrode was developed by the electrochemical polymerization, and ssDNA was successfully immobilized on the modified electrode. The preparation conditions of the modified electrode and the immobilization and hybridization conditions of DNA were studied. By cyclic voltammetric detection of the redox peaks current of methylene blue intercaleted between base pairs of dsDNA, complementary ssDNA segment could be recognized and detected, thus, the detection of the different DNA segment in the solution could also be realized.

关 键 词：电化学传感器 DNA 聚苯胺 亚甲基蓝 

分 类 号：O657.15[理学—分析化学]