RIG-Ⅰ与干扰素信号通路的关系  

Relationship between RIG-Ⅰ and IFNs signaling pathway

作　　者：孙岳平[1] 肖家祁[2] 邹嫣琼[1] 王铸钢[3] 

机构地区：[1]上海交通大学基础医学院细胞生物学教研室,上海200025 [2]上海交通大学基础医学院病原生物学教研室,上海200025 [3]上海交通大学基础医学院医学遗传学教研室,上海200025

出　　处：《上海交通大学学报（医学版）》2007年第10期1210-1214,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的研究干扰素(IFNs)对维甲酸诱导Ⅰ型基因(RIG-Ⅰ)的调控作用,同时探讨RIG-Ⅰ对IFNs生物学效应的介导作用。方法Northern杂交和半定量RT-PCR检测小鼠胚胎成纤维细胞(MEFs)、在IFNs作用前后RIG-Ⅰ基因的表达。MTT比色法和细胞病变抑制法比较野生型和RIG-基因剔除(RIG-Ⅰ-/-)小鼠的MEFs在IFNs作用前后的生长情况和抗病毒活性。结果IFN-γ、IFN-β均能上调小鼠MEFs中RIG-Ⅰ的表达;100 U/mL IFN-γ处理后,野生型小鼠MEFs的生长抑制较RIG-Ⅰ-/-小鼠的MEFs明显;RIG-Ⅰ缺失后,IFN-β对细胞病毒感染的保护作用较野生型明显减弱。结论RIG-Ⅰ是一新发现的介导干扰素细胞效应的基因,它可能通过调节细胞因子的产生参与干扰素介导的炎症反应。Objective To study the regulatory effect of interferons （IFNs） on retinoic acid-inducible gene-Ⅰ （ RIG-Ⅰ ） and the roles of RIG-Ⅰ in IFNs signaling pathway. Methods RIG-Ⅰ expression before and after IFNs treatment in mouse embryonic fibroblasts （MEFs） were analyzed with Northern blotting and semi-quantitative RT-PCR assay. MEFs isolated from wild-type and RIG-Ⅰ^-/- mice were used to test growth inhibition and antiviral activity of IFNs with MTT assay and cytopathic effect inhibition assay. Results Both IFN-γ, and IFN-β could induce RIG-Ⅰ expression in MEFs. Treated with 100 U/mL IFN-γ, growth inhibition and antiviral activity of MEFs from wild-type mice were more significant than those from RIG-Ⅰ^ -/- mice. With the absence of RIG-Ⅰ , the antiviral protective role IFN-β plays was significantly weaker than the wild type. Conclusion RIG-Ⅰ gene is a novel mediator of interferon effects on cells. It may participate in the inflammation responses mediated by IFNs through modulating cytokines production.

关 键 词：维甲酸诱导Ⅰ型基因 干扰素 炎症反应 

分 类 号：R33[医药卫生—人体生理学]