胰高血糖素样肽-1抑制软脂酸诱导的MIN6细胞凋亡  

Glucagon like peptide-1 inhibits apoptosis of MIN6 cell lines induced by palmitic acid

作　　者：姜苏原[1] 李小英[1] 宁光[1] 王卫庆[1] 

机构地区：[1]上海交通大学医学院瑞金医院内分泌代谢病科上海市内分泌代谢病临床医学中心,上海200025

出　　处：《上海交通大学学报（医学版）》2007年第10期1215-1217,共3页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的探讨胰高血糖素样肽-1(GLP-1)对软脂酸(PA)诱导的MIN6细胞凋亡及分泌胰岛素功能的影响。方法传代培养处于对数生长期的胰岛β细胞MIN6细胞株分为三组。PA组:加入0.5 mmol/L PA孵育36 h;GLP-1+PA组:加入10 nmol/LGLP-1,12 h后再加入0.5 mmol/L PA继续孵育36 h,期间每12 h加1次相同浓度的GLP-1;对照组:未加GLP-1或PA,其他培养条件相同。MTT比色法检测细胞活力;Annexin V-FITC/PI双染流式细胞术检测细胞凋亡百分率;放射免疫分析法测定MIN6细胞胰岛素分泌量。结果PA组MIN6细胞凋亡率为(39.41±10.16)%,细胞活力较对照组减少25.8%(P<0.05);GLP-1+PA组MIN6细胞凋亡率为(32.80±8.06)%,细胞活力较PA组增强13.88%(P<0.05),其高糖和低糖状态下胰岛素分泌均明显高于PA组(P<0.05)。结论GLP-1可以抑制PA诱导的MIN6细胞凋亡,同时改善细胞胰岛素分泌功能。Objective To investigate the effects of glucagon like peptide-1 （GLP-1） on palmitic acid （PA）-induced apoptosis of MIN6 cell lines and insulin secretion of MIN6 cell lines. Methods The MIN6 cell lines of pancreatic 13 cells under serial subcuhivation were divided into three groups according to the treatment. PA group： 0.5 mmol/L PA for 36 h; GLP-1 ＋PA group： 10 nmol/L GLP-1 for 12 h and then 0.5 mmol/L PA for 36 h, with GLP-1 of same concentration per 12 h ; control group： no GLP-1 or PA, with the same other condition. Cell viability was determined by MTT chromatometry, the percentage of apoptosis was measured by flow cytometry with propidium iodide and Annexin V-FITC staining, and the insulin concentration in media was detected by radioimmunoassay. ResultsThe apoptosis rate of MIN6 cell lines in PA group was （39.41 ±10.16） % , and the cell viability decreased 25.8% compared with control group（ P 〈 0.05）. The apoptosis rate of MIN6 cell lines in GLP-1 ＋ PA group was （32.80 ± 8.06）% , the cell viability increased 13.88% compared with PA group（P 〈0.05）, and the insulin secretion was significantly higher than that of PA group in both low and high glucose concentration（P 〈0.05）. Conclusion GLP-1 can inhibit PA-induced apoptosis on MIN6 cell line and improve the insulin secretion function.

关 键 词：胰高血糖素样肽-1 软脂酸 凋亡 MIN6细胞 胰岛素 

分 类 号：R587.1[医药卫生—内分泌]