RP-HPLC测定活血止痛片中三七皂苷R_1和人参皂苷Rg_1含量  

Quantitative determination of notoginsenoside R_1 and ginsenoside Rg_1 in Huoxue Zhitong tablet by RP-HPLC method

作　　者：陈再兴[1] 袁长季[1] 李丽红[1] 刘丽[2] 郝艳玲[3] 姜泓[1] 

机构地区：[1]中国医科大学药学院,北京110001 [2]辽宁中医药大学,沈阳110032 [3]沈阳市骨科医院,沈阳110032

出　　处：《天津中医药》2007年第5期414-415,共2页Tianjin Journal of Traditional Chinese Medicine

摘　　要：[目的]建立活血止痛片中三七皂苷R1和人参皂苷Rg1的含量测定方法。[方法]采用反相高效液相色谱法(RP-HPLC)。[结果]三七皂苷R1的线性范围为0.207～1.036μg,人参皂苷Rg1的线性范围为0.829～4.148μg,样品平均回收率分别为96.1%,97.3%,RSD分别为2.02%,2.34%。[结论]本方法可准确测定活血止痛片中三七皂苷R1和人参皂苷Rg1的含量。[Objective] To perfect the quality standard of notoginsenoside R1 and ginsenoside Rg1 in the Huoxue Zhitong tablet. [Methods] The chromatographic separation was performed on a Kromasil C18 column with a linear gradient elution of acetonitrile-0.05% phosphoric acid, 0-10min, 20% acetonitrile; 10-40min, 20% acetonitrile -30% acetonitrile. Detection wavelength was set at 203 nm; flow rate was 1.0 mL/min and column temperature was set at 30℃. [Results] Notoginsenoside R1 and ginsenoside Rg1 had a good linearity in the range of 0.207 2-1.036 μg and 0.8296-4.148μg, The average recovery of this method was 96.1%, 97.3%, RSD=2.02%, 2.34%（n=5）respectively. [Conclusions] The procedure is simple and reliable and the results were stable and reproducible. The established method can determine notoginsenoside R1 and ginsenoside Rg1 in the Huoxue Zhitong tablet accurately.

关 键 词：活血止痛片 反相高效液相色谱法 三七皂苷R1 人参皂苷RG1 

分 类 号：R285.5[医药卫生—中药学]