单独或联合应用酸性成纤维细胞生长因子与转化生长因子对猪牙乳头细胞增殖的影响  

Effects of acidic fibroblast growth factor and transforming growth factor-β1 on proliferation of porcine dental papilla cells

作　　者：余姗姗[1] 蒋欣泉[1] 翁雨来[1] 花菲[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔内科,上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011

出　　处：《口腔医学》2007年第7期347-350,共4页Stomatology

基　　金：国家重点基础研究发展规划973项目(G1999054308)

摘　　要：目的研究酸性成纤维细胞生长因子（aFGF）和转化生长因子（TGF）β1单独或联合应用后对猪牙乳头细胞（pDPCs）增殖的影响。方法通过体外细胞培养技术,用四甲基偶氮唑盐（MTT）比色法检测aFGF、TGFβ1不同浓度、不同时间单独及联合应用后对pDPCs增殖的影响,所得数据用单因素方差进行分析。结果aFGF在5~100ng/ml和8d范围内能促进pDPCs增殖,最强效应浓度为10ng/ml和25ng/ml,最强效应时间为8d;TGFβ1在5~50ng/ml和8d范围内能抑制pDPCs增殖,其中以5ng/ml和10ng/ml抑制作用较弱;以aFGF最强效应浓度与TGFβ1较弱抑制浓度交互联合后对pDPCs有显著的促进增殖作用,其中以aFGF25ng/ml＋TGFβ110ng/ml的效果最明显。Objective To investigate the effects of acidic fibroblast growth factor and transforming growth factor-β1 on the proliferation of porcine dental papilla cells. Methods Isolated porcine dental papilla cells were cultured in DMEM/F12 supplemented with aFGF and TGF β1. MTF colorimetric method was used to assess dose-effect and time-effect of aFGF and TGF β1 on the proliferation of pDPCs. All statistical analyses were performed with one-way ANOVA. Results Within 8 days, aFGF could significantly promote the proliferation of pDPCs with the concentrations between 5 ng/ml and 100 ng/ml, especially with the concentration of 10 ng/ml and 25 ng/ml, while the proliferation effects of TGF β1 sharply decreased at the dose varying from 5 ng/ml to 50 ng/ml. The effect was the strongest for the proliferation of pDPCs with the combination of 25 ng/ml aFGF and 10 ng/ml TGF β1. Conclusion The results suggested that in certain conditions, aFGF enhanced the proliferation of pDPCs while TGF β1 inhibited the proliferation . However,the promotive effects were achieved when the growth factors were used in combination. Supported by National 973 Project （ G1999054308 ）.

关 键 词：牙乳头细胞 酸性成纤维细胞生长因子 转化生长因子Β1 

分 类 号：R349.53[医药卫生—基础医学]