分光光度法测定血液及脑组织中环己亚硝脲  

Colorimetric determination of CCNU in blood and brain tissue

作　　者：田新华[1,2] 江澄川[1,2] 张其清 任磊[1,2] 刘玲蓉 王福君 

机构地区：[1]上海医科大学华山医院脑神经外科 [2]中国医学科学院中国协和医科大学生物医学工程所生物材料研究室

出　　处：《中国药学杂志》1997年第4期232-234,共3页Chinese Pharmaceutical Journal

摘　　要：目的：测定血液和脑组织中微量环己亚硝脲（ＣＣＮＵ）。方法：利用ＣＣＮＵ在过量酸中的脱亚硝基反应，用分光光度法测定，对实验数据进行了ＳＡＳ软件处理。并进一步分析讨论了该方法的可靠性和应用范围。结果：血液及脑组织中药物浓度与吸光度间线性相关良好，二者的测试敏感度分别为０．２μｇ·ｍｌ－１和０．４μｇ·ｍｌ－１。结论：采用本方法测定血液及脑组织中的微量ＣＣＮＵ含量是可行的。OBJECTIVE: To determine the CCNU concentration in blood and brain tissue. METHODS: The ready liberation of nitrous acid by CCNU in hydrochloric acid solution was used successfully in the development of a sensitive method for its quantitative estimation in biological body. RESULTS: The linear regressions between CCNU concentration of blood, brain tissue and absorbance were significent. CONCLUSION: The method for determining the CCNU concentrtion is feasible. Some valuable experimental data are proposed for practical application.

关 键 词：环己亚硝脲 分光光度法 测定 抗癌药 

分 类 号：R979.1[医药卫生—药品] R927.2[医药卫生—药学]