高效液相色谱法测定盐酸洛美沙星葡萄糖注射液的含量  

HPLC for concentration determination of lomefloxacin hydrochloride and glucose injection

作　　者：谷杰[1,2] 初文英 李铁军 杨德君[1,2] 

机构地区：[1]吉林化学工业公司医院临床药物研究室 [2]吉林伊通医药生物技术研究所

出　　处：《中国药学杂志》1997年第4期234-235,共2页Chinese Pharmaceutical Journal

摘　　要：目的：探索准确而简便地测定盐酸洛美沙星葡萄糖注射液的含量。方法：采用高效液相色谱法，用硅胶柱，以十二烷基硫酸钠磷酸二氢钾混合溶液甲醇（６０∶４０）为流动相，在波长２８７ｎｍ处进行检测。结果：在５．５５～１１１．００μｇ·ｍｌ－１的浓度范围具有良好的线性关系，ｒ＝０．９９９８，平均回收率９９．５９％（ｎ＝５），ＲＳＤ＝０．５４％。结论：本法适用于成品检验及杂质或降解产物的检查。OBJECTIVE: To find a precise, simple and convenient method for concentration determination of lomefloxacin hydrochloride and glucose injection. METHOD: A HPLC method was chromatographed on silica columns with a mobile phase of a mixture solution of sodium dodecyl sulphate and potassium dihydrogen phosphate methanol(60∶40) with detection at wave length 287 nm. RESULTS: The linear range was 5.55～111.00 μg·ml -1 , the average recovery 99.59%, and RSD 0.54%(n=5). CONCLUSION: The method can be used to determine finished product and impurity.

关 键 词：盐酸 洛美沙星 葡萄糖注射液 高效液相色谱 

分 类 号：R978.19[医药卫生—药品] R927.2[医药卫生—药学]