谷氨酸对PC12细胞中磷酸化肌细胞增强因子2A蛋白表达的影响  

Effects of Glutamate on the Expression of Phosphorylated Myocyte Enhancer Factor 2A Protein in PC12 cells

作　　者：薛梅[1] 秦小红[1] 陈吉庆[2] 许万宏[1] 周国平[2] 周艳[2] 陆超[2] 

机构地区：[1]泰州市人民医院儿科,江苏泰州225300 [2]南京医科大学第一附属医院儿科,南京210029

出　　处：《实用儿科临床杂志》2007年第19期1482-1483,共2页Journal of Applied Clinical Pediatrics

基　　金：江苏省卫生厅医学科技发展基金项目资助(K200504);泰州市社会发展基金项目资助(泰科2006-172)

摘　　要：目的研究兴奋性氨基酸谷氨酸对PC12细胞中磷酸化肌细胞增强因子2A(P-MEF2A)蛋白表达的影响与意义。方法体外培养PC12细胞,设立正常对照组,0.1、1.0、10.0 nmol/L谷氨酸处理组。Western blot测定P-MEF2A与突触素1的蛋白表达量,并进行2种蛋白表达相关性分析。结果谷氨酸诱导PC12细胞中P-MEF2A蛋白表达升高,呈剂量依赖性(F=51.54 P<0.01)。10.0 nmol/L谷氨酸处理24 h后,P-MEF2A蛋白的表达与突触素1的表达量呈显著负相关(r=-0.788 P<0.01)。结论谷氨酸诱导PC12细胞P-MEF2A表达升高,并与学习记忆调控相关基因突触素1的表达下调有关。Objective To investigate the effects of glutamate on the expression of phosphorylated myocyte enhancer factor 2A（ P - MEF 2A） protein in PC12 cells. Methods PC12 cells were incubated and were divided into normal group, 0.1 nmol/L, 1.0 nmol/L, 10.0 nmol/L glutamate treated group. Western blot was used to detect the protein levels of P - MEF2A and synapsin - 1. Results Glutamate induced the increased of P- MEF2A protein levels in a dose -depended way （ F =51.54 P 〈0.01）. After PC12 cells were exposed to 10.0 nmol/L glutamate for 24 h, the expression levels of P - MEF2A protein were significantly inverse correlated with synapsin - 1 protein levels （ r = - 0. 788 P 〈0.01 ）. Conclusions Glutamate induced the increase of P - MEF2A in PC12 cells. And it might be correlated with the decrease of synapsin - 1 which is related with learning and memory.

关 键 词：谷氨酸 肌细胞增强因子2A 突触素1 

分 类 号：R722.1[医药卫生—儿科]