NIDDM 中脂蛋白脂酶基因多态性的研究  

POLYMORPHISM IN THE LIPOPROTEIN LIPASE GENE IN PATIENTS WITH NIDDM

作　　者：朱丁达 孙荣[1,2] 高梅[1,2] 郑以漫 李小英[1,2] 赵列宾 许曼音[1,2] 陈家伦 

机构地区：[1]上海第二医科大学附属瑞金医院 [2]上海市内分泌研究所

出　　处：《中华内分泌代谢杂志》1997年第2期73-76,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：为探讨脂蛋白脂酶基因变化与ＮＩＤＤＭ并发症的关系，我们采用聚合酶链反应和限制性片段长度多态性方法，对１０２例ＮＩＤＤＭ患者和６２例正常对照者脂蛋白脂酶基因８号内含子作了多态性分析。结果表明含ＨｉｎｄＩＩ酶切位点的“＋”等位基因和不含酶切位点的“－”等位基因在ＮＩＤＤＭ组和对照组分布频率分别为“＋”０．８０、０．８１和“－”０．２０、０．１９。三类基因型的分布各为＋／＋６３．７％、６７．７％，＋／－３４．３％、２７．４，－／－２％、４．９％。两组人群间在基因型和等位基因型上均无显著差异。在ＮＩＤＤＭ患者中，＋／＋基因型患者的血甘油三酯水平明显高于非＋／＋基因型（Ｐ＜０．０５），而在其他血脂水平上，以及ＮＩＤＤＭ并发症的发生率上，各基因型之间均无显著差别。提示脂蛋白脂酶基因变化只是导致ＮＩＤＤＭ并发症的原因之一。For studying the relationship between the lipoprotein lipase gene and cardiovascular complications of NIDDM,the polymorphism of the 8th intron of lipoprotein lipase gene was determined with the PCR and RFLP methods,in 102 NIDDM patients and 62 normal people as control. The frequency of the allele with the restriction site of Hind III(“+”) was compared with the allele without the restriction site (“-”). The frequencies between NIDDM and the control group were respectively 0.80,0.81 and 0.20,0.19. The frequencies of genotypes between them were +/+ 63.7% and 67.7%,+/- 34.3% and 27.4% and -/- 2% and 4.9%, respectively. There was no significant difference of frequencies between the two groups either in genotypes or in alleles. In the NIDDM group, patients with +/+ genotype had a higher plasma triglyceride value than those with the other genotypes (including +/- and -/- genotypes)(P<0.05). There was no significant difference in the plasma total cholesterol, HDL-C,LDL-C, ApoA, ApoB, Lp(a) and in the incidence of the diabetic complications such as obesity, hypertension, coronary heart disease, etc. These results suggest that change in lipoprotein lipase gene is only one of the couses leading to NIDDM complications.

关 键 词：糖尿病 IDDM 脂蛋白脂酶 基因多态性 

分 类 号：R587.102[医药卫生—内分泌]