大鼠胸主动脉球囊损伤后早期平滑肌细胞凋亡观察  

THE EARLY APOPTOSIS OF SMOOTH MUSCLE CELLS OF THORACIC AORTA IN POST-BALLOON-INJURY RATS

作　　者：褚现明[1] 李冰[2] 高美华[3] 董果雄[4] 

机构地区：[1]青岛大学医学院附属海慈医院心内科,山东青岛266033 [2]青岛大学医学院生物学教研室,山东青岛266033 [3]青岛大学医学院免疫学教研室,山东青岛266033 [4]青岛大学医学院附属医院心内科,山东青岛266033

出　　处：《青岛大学医学院学报》2007年第6期517-519,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的探讨大鼠胸主动脉球囊损伤后早期细胞凋亡和凋亡相关基因表达的变化及其规律。方法将36只雄性SD大鼠随机分为两组,手术组(n=30)行球囊扩张损伤大鼠胸主动脉术;对照组(n=6)不行球囊损伤。取正常及损伤术后即刻、30min、1h、2h、4h胸主动脉,应用苏木精-伊红染色、TUNEL法、免疫组化和计算机图像分析等方法进行形态学观察,进行细胞凋亡、凋亡基因Fas、抗凋亡基因Bcl-2表达水平检测。结果正常大鼠胸主动脉管腔面有单层完整内皮细胞,内外弹力层呈波浪形,完整连续;手术组球囊损伤后可见内弹力层模糊,多处断裂,管腔面毛糙无内皮细胞;损伤后4h中膜平滑肌细胞密度较对照组减少。对照组、手术组损伤后即刻胸主动脉偶见凋亡细胞;手术组球囊损伤后30min,管壁中膜可见大量凋亡平滑肌细胞,损伤后1、2h中膜凋亡细胞明显减少,损伤后4h中膜仅偶见凋亡细胞。对照组、手术组动脉损伤后即刻胸主动脉偶见Fas阳性平滑肌细胞,球囊损伤后30min中膜可见大量Fas阳性细胞,损伤后1、2h中膜Fas阳性细胞明显减少,动脉损伤后4h仅偶见Fas阳性平滑肌细胞。对照组动脉壁显著表达Bcl-2,手术组胸主动脉中膜Bcl-2表达迅速下降,损伤后1h达最低水平,后回升。Bcl-2与Fas表达呈负相关(r=-0.816,P<0.01)。结论平滑肌细胞凋亡可能在动脉损伤后平滑肌细胞过度增殖和迁移过程中具有重要作用。Objective To investigate the early changes of apoptosis and the expression of Fas/Bcl-2 in rat aorta after balloon withdrawal injury. Methods Thirty-six SD rats were randomly divided into two groups： operation group （n=30） and control group （n=6）. The rats in operation group had balloon induced endothelium denudation in thoracic aorta and were killed at the time of balloon in iury and 0.5, 1, 2, and 4 hours later. Using TUNEL labeling, immunohistochemistry, and computerized image analysis to observe the aorta for smooth muscle cell apoptosis, the expression of Fas/Bcl- 2 and the morphology. Results The surface of vessel lumen of normal rat aorta had a monolayer of integrated endothelial cells. The internal elastic lamina was ruptured after the injury, and no endothelial cells were found. The density of vascular smooth muscle cells was reduced obviously 4 hours later. Few apoptotic cells were detected in the uninjured control vessels and in vessels immediately after injury. Remarkable medial vascular smooth muscle cells appeared apoptotic at 30 min, and then significantly reduced at 1 or 2 hours, and were occasionally seen 4 hours later. In normal rat arteries, Eel-2 was detected after balloon injury, its expression in media reached the lowest level at 1 hour and thenupregulated. The expression of Fas and Bcl-2 showed remarkably negative correlation （r=-0.816,P〈0.01）. Conclusion The changes of apoptosis of smooth muscle cells probably play an important role in the process of excessive proliferation and transfer of the muscle cells after artery injury.

关 键 词：肌细胞 平滑肌 血管 凋亡 FAS BCL-2 球囊损伤 大鼠 

分 类 号：R541.4[医药卫生—心血管疾病]