应激致免疫功能降低动物模型的建立  

Establishment of an Animal Model of Stress-Induced Immune Dysfunction

作　　者：侯殿东[1] 赵宝霞[1] 刘辉[1] 

机构地区：[1]大连医科大学临床免疫学教研室,大连116027

出　　处：《中国实验动物学报》2007年第5期330-332,I0004,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金资助项目(编号:30370484)

摘　　要：目的为探讨应激对免疫系统损伤的作用机制,建立了两种不同的应激导致机体免疫功能降低的动物模型。方法将小鼠分为三组,束缚应激组、热应激组和正常对照组,分别用束缚和高温刺激使小鼠处于应激状态,应激结束后将各组小鼠均分为两部分,一部分感染细菌,比较两种应激状态下小鼠与正常小鼠的死亡率,另一部分摘取脾脏,计算脾脏重量指数,制作脾脏病理切片,观察其组织病理学变化。结果注射大肠埃希菌后束缚应激组和热应激组小鼠死亡率显著高于正常对照组小鼠(P<0.005),束缚应激组和热应激组小鼠死亡率无明显差异(P>0.05)。束缚应激组小鼠和热应激组小鼠脾脏重量指数均较正常对照组明显降低(P<0.05),组织病理学观察显示束缚应激组和热应激组小鼠脾脏切片病理学改变相似,白髓比例减小,脾小体有不同程度的缩小、分布及结构不规则。结论本实验建立的应激导致小鼠免疫功能降低的动物模型,其免疫功能降低与脾脏变化间存在构效关系,与应激的类型关系不大。Objective In order to explore the mechanism of stress-induced immune dysfunction, two kinds of animal models with depressed immune response under stresses were established. Methods The mice were divided into 3 groups： the restraint-stressed group, heat-stressed group and control group. The experimental mice were stressed by restraint and heat, respectively. After being stressed, the mice of each group were subdivided into two parts, one part was injected by Escherichia coli. The death rate of each group was calculated. In other of them, spleen indexes of the mice of each group were compared, and the histopathological changes were examined. Results The death rates of the restrained stress group and heat-stressed group were all higher than that of control group （ P 〈 0.005）, The death rates were not significantly different between the restrained stress group and heat-stressed group （P 〉 0.05） . The spleen indexes of both the restraint stress group and heat-stress group were lower than that of the control group （P 〈 0.05）. The histopathological examination revealed white pulp decreased with disarrangement in spleen of stressed mice. Conclusion The mice models of stress-induced immune dysfunction have been successfully established and there may be a correlation between the spleen structural alterations and immune dysfunction. The changes are not significantly related to types of stresses.

关 键 词：动物模型 应激 免疫功能降低 

分 类 号：O3[理学—力学]