(GTG)_5探针产生的DNA指纹图在近交系小鼠遗传检测中的应用  

Application of DNA Fingerprinting Generated by(GTG)5 Probe in Genetic Monitoring of Inbred Strain Mice

作　　者：王芳[1] 孙以方[1] 段天林[1] 安蓓[1] 张文慧[1] 

机构地区：[1]兰州大学医学实验中心,兰州730000

出　　处：《中国实验动物学报》2007年第5期361-364,共4页Acta Laboratorium Animalis Scientia Sinica

摘　　要：目的探讨用非放射性标记的寡聚核苷酸探针（GTG）5进行近交系小鼠的DNA指纹分析。方法用非放射性标记的寡聚核苷酸探针（GTG）5制作BALB/c、C57BL/6J、DBA/2、C3H近交系小鼠的DNA指纹图,对这四个品系的小鼠进行遗传检测,分析各品系内和品系间的遗传变异性。结果（GTG）5探针可产生具有良好多态性的DNA指纹图,平均图带数为8-12条。各品系内的DNA指纹图平均相似系数（^-x）在0.96-1.00的范围内,具有相同指纹图的概率（P）均在3.1×10^-1以上,极显著地高于品系间的相似系数（0.22-0.39）和相同指纹图的概率（P〈1.07×10^-4）。结论（GTG）5可用于制作近交系小鼠的DNA指纹图以对其进行遗传检测。Objective To investigate the DNA fingerprinting of inbred strain mice generated by non-radioactive labeled oligonucleotide probes （GTG）5. Methods DNA fingerprints of BALB/c, C57BL/6J, DBA/2, C3H inbred strain mice were generated by non-radioactive labeled oligonucleotide probes （GTG）5 to make a genetic monitoring for these four breeds and to determine the intrabreed and interbreed variations of mouse DNA fingerprints. Results There were 8 - 12 distinguishable bands on every DNA fingerprinting map and each map had polymorphism, The similarity coefficient （^-x） within breeds was 0.96 - 1.00, the probability of an identical DNA fingerprinting in them （ P ） was above 3.1×10^-1 , significantly higher than that between breeds （^-x = 0.22 - 0.39, P 〈 1.07×10^-4 ）. Conclusion The oligonucleotide probe （GTG） 5 could be used in DNA fingerprinting for genetic monitoring in inbred strain mice.

关 键 词：寡聚核苷酸探针 DNA指纹法 小鼠 近交系 遗传检测 

分 类 号：S813.23[农业科学—畜牧学] Q78[农业科学—畜牧兽医]