江西地方猪种SLA-DRB基因外显子2的多态性研究  

Polymorphism analysis of the exon 2 of SLA-DRB gene in three native pig breeds of Jiangxi province

作　　者：鞠慧萍[1] 吴圣龙[1] 包文斌[1] 程金花[1] 李碧春[1] 陈国宏[1] 杨建生[2] 黄雪根 华金弟 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省昆山市畜牧兽医站,江苏昆山215300 [3]江苏省苏州市苏太猪育种中心,江苏苏州215128

出　　处：《畜牧与兽医》2007年第7期4-6,共3页Animal Husbandry & Veterinary Medicine

基　　金：江苏省高技术研究发展计划项目(BG2006302);扬州大学自然科学基金资助项目(NK0513119)

摘　　要：采用PCR-RFLP法对3个江西地方猪种(乐平花猪、修水杭猪和万安花猪)的SLA-DRB基因外显子2的PCR产物进行多态性分析。通过限制型内切酶RsaⅠ酶切后,共产生9种组合带型。通过检测发现:乐平花猪有8种组合带型,即AA、AB、AC、BB、BC、BD、CD和DD;修水杭猪有3种组合带型,即AA、AB和AC;万安花猪有4种组合带型,即AA、AC、BC和CC,且3个猪种中均以A为优势带型,在猪种中频率相差不大。χ2检测表明,乐平花猪和修水杭猪SLA-DRB基因外显子2在RsaⅠ酶切位点达到了Hardy-Weinberg平衡状态,而万安花猪未达到Hardy-Weinberg平衡状态。The exon 2 of SLA-DRB gene in three native pig breeds of Jiangxi province was analysed by PCR-RFLP method. The results showed that the exon 2 digested by Rsa I could be divided into 9 kinds of combined patterns. All combined patterns CC were observed in Leping Spotted pig; AA, AB and AC in Hang pig, AA, AC, BC and CC in Wanhn Spotted pig. In each breed, A was the predominant pattern, and there were no significant differences in the frequency of A among three breeds. The results of Chi Square test indicated that the frequencies of patterns digested by Rsa I were Hardy-Weinberg equilibrium in Leping Spotted and hang pig populations （ P 〉 O. 05 ）.

关 键 词：地方猪种 SLA-DRB PCR-RFLP 

分 类 号：S828[农业科学—畜牧学]