氧氟沙星对映体的毛细管电泳手性分离  

CHIRAL SEPARATION OF OFLOXACIN ENANTIOMERS BY CAPILLARY ELECTROPHORESIS

作　　者：俞犄[1] 王义明[1] 罗国安[1] 

机构地区：[1]清华大学化学系

出　　处：《药学学报》1997年第3期203-206,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：用高效毛细管电泳和二极管阵列检测器，以环糊精为拆分试剂对氧氟沙星进行手性分离（最大分离度可达２１８）。发现衍生化环糊精有较好的分离效果，用含ＤＭβＣＤ４０ｍｍｏｌ·Ｌ－１，ＫＨ２ＰＯ４７０ｍｍｏｌ·Ｌ－１，ｐＨ２５的缓冲液，在２５℃电泳，２０ｋＶ分离电压下得到优化的分离结果。A method was developed to separate the enantiomers of ofloxacin, one kind of broad spectrum antibiotics, by capillary electrophoresis with derivatized cyclodextrins (CDs) as chiral selector. The separation was performed on a 57 cm×75 μm uncoated capillary. The optimal composition of mobile phase was 40 mmol·L -1 DM β CD(dimethyl β cyclodextrin) in pH 2 5 phosphate buffer. The separation factor of enantiomers was 2 18. A diode array detector was employed for the detection of enantiomers. The effects of CD concentration in buffer, the electrolyte concentration, temperature and separation potential were also investigated.

关 键 词：毛细管电泳 手性分离 氧氟沙星 抗菌药 

分 类 号：R978.19[医药卫生—药品]