核桃楸叶乙醇提取物的抑菌活性及活性成分分析  

Antifungal Activities and Active Components of Alcoholic Extracts from Leaves of Juglans mandshurica Maxim.

作　　者：孙墨珑[1] 宋湛谦 方桂珍[4] 

机构地区：[1]东北林业大学理学院,黑龙江哈尔滨150040 [2]中国林业科学研究院林产化学工业研究所 [3]国家林业局林产化学工程重点开放性实验室,江苏南京210042 [4]东北林业大学材料科学与工程学院,黑龙江哈尔滨150040

出　　处：《林产化学与工业》2007年第B10期81-84,共4页Chemistry and Industry of Forest Products

基　　金：哈尔滨市留学回国人员基金项目(2005AFLXJ005)

摘　　要：采用常规提取法浸提核桃楸叶的抑菌活性成分。研究结果表明,核桃楸叶乙醇提取物及其乙酸乙酯萃取相对杨树叶枯病菌和樟子松枯梢病菌有抑菌活性,核桃楸叶乙醇提取物及其乙酸乙酯萃取相对杨树叶枯病菌的最低抑菌质量浓度(MIC)为3.12 g/L,对樟子松枯梢病菌的MIC为6.25 g/L。采用GC-MS分析了核桃楸叶乙醇提取物中乙酸乙酯萃取相经柱层析后集分I和集分II的化学组成和相对含量。分析结果表明,乙酸乙酯萃取相中主要活性成分为相对含量较高的5-羟基-1,4-萘醌(胡桃醌)、D-阿洛糖、5,8-二羟基-1,4-萘醌、1,5-萘二酚、2,3-二氢苯并呋喃、7-甲氧基-1-四氢萘酮、1-萘酚、8-羟基-2-甲氧基-1,4-萘醌和4-甲基-2,6-二羟基喹啉等。The extracts from leaves of Juglans mandshurica Maxim. were obtained by common extracting method for studying its antifungal activities and analyzing the active components. The results showed that alcoholic extracts and the acetic ether part of alcoholic extracts （ separated with acetic ether from alcoholic extracts） from leaves of J. mandshurica had antifungal activities toward Alternarla alternate Fr. and Sphaeropsis sapinea Fr.. MIC of the alcoholic extracts and the acetic ether part of alcoholic extract were both 3.12 g/L toward A. alternate, and both 6.25 g/L toward S. sapinea. The active components in the acetic ether part I and part Ⅱ （ separated with silica gel column chromatography） of alcoholic extracts from leaves of J. mandshurica were analyzed by GC-MS. The results showed that major active components of relatively high content in the acetic ether part of alcoholic extracts from leaves of J. mandshurica were 1,4-naphthalenedione, 5-hydroxy-（juglone）, D-allose, 5,8-dihydroxy-1,4- naphthalenedione, 1,5-naphthalenediol, 2,3-dihydro-benzofuran, 7-methoxy-l-tetralone, 1-naphthalenol, 8-hydroxy-2-methoxy- 1,4-naphthalenedione and g-methyl-2,6-dihydroxyquinoline.

关 键 词：核桃楸叶 提取物 抑茵活性 

分 类 号：TQ91[化学工程]