蜂胶乔松素对脂多糖诱导人脐静脉内皮细胞的保护作用  

Protective effect of pinocembrin from propolis on human umbilical vein endothelial cells induced by lipopolysaccharide

作　　者：李东娟[1] 王家富[1] 李伟[1] 

机构地区：[1]泰山医学院病理学教研室,山东省泰安市271000

出　　处：《中国组织工程研究与临床康复》2007年第41期8318-8320,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:观察蜂胶乔松素对脂多糖诱导人脐静脉内皮细胞的影响,探讨其对人脐静脉内皮细胞可能的保护作用。方法:实验于2006-03/10在泰山医学院生命科学研究所(省重点实验室)完成。①实验材料:取出生1h内新生儿脐带,患者知情同意。②实验分组及方法:培养人脐静脉内皮细胞,建立脂多糖损伤模型(以10mg/L的脂多糖培养液培养细胞12h),实验分为空白对照组(加等量D-Hank’s液)、脂多糖组(10mg/L)、乔松素组(加10mg/L脂多糖预孵育12h后,按50,100,200mg/L分别加入乔松素),各组设8个复孔,共同孵育24h。③实验评估:光镜下观察细胞形态,MTT法观察乔松素对人脐静脉内皮细胞活性的影响,ELISA方法检测培养上清中血管假血友病因子的含量,TUNEL检测细胞凋亡率。结果:①细胞形态:空白对照组细胞紧密贴壁,呈铺路石状生长。脂多糖组可见多数细胞呈圆形;乔松素组见上述细胞较脂多糖组明显减少。②乔松素对人脐静脉内皮细胞活性、凋亡及血管假血友病因子含量的影响:与对照组比较,脂多糖组能明显诱导人脐静脉内皮细胞的凋亡(P<0.01),不同浓度乔松素组可改善内皮细胞形态,组织活性明显升高(P<0.05),同时抑制内皮细胞血管假血友病因子的释放(P<0.05),使脂多糖诱导的人脐静脉内皮细胞凋亡细胞数明显减少(P<0.05)。结论:乔松素能增强人脐静脉内皮细胞活性,抑制脂多糖诱导人脐静脉内皮细胞的凋亡,从而发挥可能的内皮细胞保护功能。AIM： To investigate the effects of pinocembnn from propolis on human umbilical vein endothelial cells （HUVECs） induced by lipopolysaccharide （LPS）, and explore its possible protective effect on HUVECs. METHODS： The experiment was conducted in the Institute of Biological Sciences, Taishan Medial College from March to October 2006. （1）HUVECs were isolated from the neonatal umbilical core in 1 hour （the informed consent was obtained from the family） and cultured. The injury model was established by incubating in 10 mg/L LPS for 12 hours. There were blank control group （D-Hank＇s solution）, LPS group （10 mg/L）, and pinocembnn groups （50, 100, and 200 mg/L after incubated in 10 mg/L LPA for 12 hours） in the study with 8 wells in. each group. （2）After treatment of 24 hours, cell morphous was observed under light microscope. Effect of pinocembnn on cell viability was assessed by MTT assay; production of von Willebrand factor （vWF） was measured by ELISA; HUVECs apoptotic rate was detected by TUNEL. RESULTS： （1）Cells in blank control group closely attached to the wall in cobble shape. Most of the cells in LPS group were round, and the number of those cells was more than that in pinocembdn group. （2）Compared with blank control group, LPS could significantly induce the apoptosis of HUVECs （P 〈 0.01）; pinocembrin at different concentrations could improve cell morphous and viability （P 〈 0.05）, inhibit the production of vWF （P 〈 0.05）, and significantly inhibit the apoptosis rate of HUVECs （P 〈 0.05）. CONCLUSION： Pinocembrin could enhance the viability of HUVECs, and inhibit the apoptosis of HUVECs, thereby it plays a role in protecting the vascular endothelial cells.

关 键 词：乔松素 蜂胶 人脐静脉内皮细胞 凋亡 脂多糖 

分 类 号：R541[医药卫生—心血管疾病]