系统性红斑狼疮与CD19基因多态性的相关性研究  

Association of CD19 gene polymorphism with systemic lupus erythematosus in Chinese

作　　者：徐安平[1] 梁艳仪[1] 吕军[1] 李劲高[1] 宛霞[1] 赖德源[1] 黄湖辉[1] 

机构地区：[1]中山大学附属第二医院肾内科,广东广州510120

出　　处：《国际内科学杂志》2007年第10期562-566,共5页International Journal of Internal Medicine

摘　　要：目的研究CD19基因第4外显子705位点(以下简称CD19基因705位点)多态性在中国南方地区汉族人群中的分布及其与系统性红斑狼疮(SLE)和狼疮肾炎(LN)的相关性。方法103例患者诊断均符合1982年美国风湿病学会修订的SLE分类标准,男13例,女90例,其中62例伴有LN。正常对照组110例,男21例,女89例。全部研究对象均为无血缘关系的中国南方地区汉族人群。应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对所有SLE患者和正常对照者进行CD19基因705位点多态性检测。结果CD19基因705位点多态性在中国南方地区汉族人群中普遍存在。SLE患者CD19基因705位点基因型和等位基因频率分布与正常对照组比较差异无统计学意义(P>0.05);CD19基因705位点基因型和等位基因频率分布,按性别分层后,男性和女性SLE患者分别与正常对照组比较及LN患者和正常对照组比较以及伴有LN患者与未伴有LN的SLE患者间比较,差异均无统计学意义(P>0.05)。结论CD19基因705位点多态性与SLE及LN无相关性。Objective To investigate the possible association of a single nucleotide polymorphism（SNP） at position 705 in exon 4 of CDI9 gene （ CD19 gene 705） in Southern Chinese patients with systemic lupus ervthe-matosus （SLE） and lupus nephritis（LN）. Methods One hundred and three patients （ 13 males and 90 females,62 cases with LN ） fulfilled the American College of Rheumatology 1982 revised criteria for SLE and I l0 healthy ethnically matched controls （21 males and 89 females）,all from the south of China, consented to participate in the study. Blood samples for DNA extraction from all subjects were collected in EDTA tube. Genomic DNA was extracted according to the standard isolation procedures. CD19 gene 705 polymorphism was typed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）. Results CDI9 gene 705 polymorphism was found widely in Southern Chinese. All genotype frequencies of CD19 gene 705 were in Hardy-Weinberg equilibrium. There were not significant differences of CDI9 gene 705 genotype , allele and phenotype frequencies between the SLE patients and the controls （ P 〉 0.05）. Similarly , no significant differences of CD19 gene 705 genotype , allele and phenotype frequencies were seen between the male ,female SLE and the controls ,the SLE with lupus nephritis（LN） and the controls, the SLE with LN and the the SLE without LN. Conclusion CD19 gene 705 polymorphism does not appear to be associated with susceptibility to SLE and LN in Southern Chinese.

关 键 词：红斑狼疮 系统性 基因 CDL9 多态性 

分 类 号：R593[医药卫生—内科学]