高效毛细管电泳测定龙胆中龙胆苦苷的含量  

HPCE determination of gentiopicroside in Radix Gentianae

作　　者：高言明[1] 宋勤 陈惠玲[1] 魏升华[1] 杨玉琴[1] 张丽艳[1] 王建科[1] 

机构地区：[1]贵阳中医学院药学系,贵阳550002 [2]贵州省药品检验所,贵阳550004

出　　处：《药物分析杂志》2007年第10期1572-1574,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：贵州省教育厅重点科研项目(黔教科2004121)

摘　　要：目的:建立采用高效毛细管电泳测定龙胆中有效成分龙胆苦苷含量的方法,为野生龙胆转为人工种植提供技术依据。方法:采用毛细管区带电泳(CZE):运行缓冲液为含25%甲醇的20 mmol·L^(-1)硼砂缓冲液(pH=9.23);工作电压30 kV;柱温25℃;检测波长:275 nm。结果:龙胆苦苷在0.06～0.48 mg·mL^(-1)的浓度范围内呈良好的线性关系(r=0.9998);平均回收率为100.5%,RSD 为1.3%。结论:该方法简便、准确,重复性好。Objective：To develop a method for determination of gentiopicroside in Radix Gentianae,and to provide the technical evidence for wild Radix Gentianae transforming into man - made planting. Method： Free solution cap- illary zone electrophoresis（CZE） were carried out in a buffer solution of 20 mmol · L^-1 borax solution containing 25 % methanol （pH 9. 23 ） ;running voltage was 30 kV;column temperature was 25 ℃ ;the detective wavelength was 275 nm. Results： Gentiopicroside showed good linearity （ r = 0. 9998 ） in the range of 0. 06 - 0. 48 mg · mL^-1. The average recovery was 100. 5% ,RSD = 1.3%. Conclusion： The method is simple, accurate and good in reproducibility.

关 键 词：高效毛细管电泳 龙胆 龙胆苦苷 

分 类 号：R917[医药卫生—药物分析学]