川产赤芍中芍药苷的含量测定  

Determination of paeoniflorin of Radix Paeoniae Rubra from Sichuan province

作　　者：全维明 黎跃成 陈先玉 蒋怀林[3] 

机构地区：[1]四川省雅安食品药品检验所,雅安625000 [2]四川省食品药品检验所,成都610036 [3]成都中医药大学,成都610072

出　　处：《药物分析杂志》2007年第10期1617-1619,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:积累川产赤芍中芍药苷含量的数据,为赤芍质量标准研究提供科学依据。方法:采用 HPLC 法,比较不同流动相对川产赤芍中芍药苷的分离效果。条件为 Kromasil-C_(18)柱(4.6mm×200mm,5μm),流动相:乙腈:水(20:80),检测波长:230nm(R≥1.5)。结果:芍药苷在0.4044～2.022μg范围内具有良好的线性关系(r=0.9999),平均回收率为98.4%,RSD 为2.0%。结论:该法操作简单、准确,可作为质量控制方法之一。Objective：To accumulate the data of determination of Paeoniflorin of Radix Paeoniae Rubra from Si- chuan province. To study its quality standards and to supply scientific data. Methods：Using the HPLC method, to compare result of separating the paeoniflofin of Radix paeoniae Rubra in different mobile phases, paeoniflorin was seperated by Kromasil C18 column（4. 6 mm×200 mm,5 μm） with a mobile phase of acetonitrile -water（20： 80） and an UV detection wavelength of 230 nm （ R ≥ 1.5 ）. Results： The calibration curve was linear in the range of 0. 4044 -2. 022 μg （r = 0. 9999）. The average recovery was 98.4% and RSD was 2. 0%. Conclusion ：The method is simple and accurate, it can be one of the quality control methods.

关 键 词：川产赤芍 芍药苷 HPLC 赤芍 

分 类 号：R917[医药卫生—药物分析学]