盐酸维拉帕米的流动注射化学发光法测定  

Flow-injection chemiluminescence determination of verapamil hydrochloride

作　　者：孟赟[1] 吕九如[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,西安710062

出　　处：《药物分析杂志》2007年第10期1640-1643,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立快速测定盐酸维拉帕米的方法。方法:在碱性介质中,M-氯代丁二酰亚胺(NCS)能与鲁米诺产生化学发光。本文发现盐酸维拉帕米对 NCS-鲁米诺化学发光反应体系有很强的增敏作用,由此建立了盐酸维拉帕米流动注射化学发光测定的新方法。结果:所建方法的线性范围为2.0×10^(-8)～1.0×10^(-6)g·mL^(-1)(r=0.9981),检出限为5×10^(-9)·mL^(-1),相对标准偏差为2.3%(C=4.0×10^(-7)g·mL^(-1),n=11)。结论:此法已用于药品盐酸维拉帕米的测定,用药典方法进行对照,结果无显著性的差异。Objeetive：A new chemiluminescence method for the determination of verapamil hydrochloride by flow injection technique was developed. Method：It was based on the chemiluminescence generated during the oxidation of luminol by N-chlorosuccinimide（NCS） in alkaline medium and verapamil hydrochloride could greatly enhance this chemiluminescence intensity. Based on this, a new flow injection chemiluminescence method of the determination of verapamil hydrochloride was and the possible mechanism for the chemiluminescence reaction was discussed. Result：The chemiluminescence intensity responded linearly to the concentration of verapamil hydrochlo- ride in the range of 2. 0 × 10^-8 - 1.0 × 10^-6 g · mL^-1 with a linear correlation of 0. 9981. The detection limit was 5 × 10^-9 g · mL^-1 verapamil hydrochloride. The relative standard deviation （RSD） for the determination of 4. 0 × 10^-8 g · mL^-1 ve those obtained by rapamil hydrochloride was 2. 3 % （ n = 11 ）. Conclusion： The results were in good agreement with method of spectrophotometry in the Chinese pharmacopoeia.

关 键 词：流动注射 化学发光 维拉帕米 鲁米诺 N-氯代丁二酰亚胺(NCS) 

分 类 号：R917[医药卫生—药物分析学]