毛细管气相色谱法测定药物保健品中六六六、滴滴涕残留量  

Determination of HCHs、DDTs residues in health products by capillary gas chromatography

作　　者：饶小思[1] 邵丽华[1] 王淑娥[1] 郭帅[1] 潘玉珍[1] 郝凤荣[1] 郭冬梅[1] 

机构地区：[1]山东大学公共卫生学院,济南250012

出　　处：《药物分析杂志》2007年第10期1663-1666,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立药用保健品中六六六、滴滴涕残留量的毛细管气相色谱分析法。方法:采用 DB-5弹性石英毛细管(30m×0.32mm×0.5μm)色谱柱,检测器为^(63)Ni 电子捕获检测器;比较分析了3种不同前处理方法对测定六六六、滴滴涕残留量的影响。结果:六六六的4种异构体和滴滴涕的4种异构体在0.5～200ng·mL^(-1)范围内线性关系良好(r>0.9990),其检出限范围为0.024～0.53ng·mL^(-1);样品的回收率为86.2%～115.9%,RSD 为1.7%～4.7%。结论:3种处理方法中正己烷-二氯甲烷-石油醚(1:2:1)依次提取效果最好。该方法简便、快速、准确,灵敏度高,适用于同时测定药用保健品中六六六、滴滴涕8种有机氯农药残留量。Objective：To establish a method of capillary gas chromatography （CGC）to quantitatively determine HCHs,DDTs in health products. Method： With gas chromatography and DB -5 flexible quartz capillary（30 m × 0. 32 mm × 0. 5 μm）chromatographic column, ^63Ni electron capture detector（ECD） ;Three kinds of different extraction methods were evaluated for the determination of HCHs,DDTs. Results：The good linearities were obtained for HCHs, DDTs （r 〉 0. 9990） and the limits of detection were between 0. 024 -0. 53 ng · mL^-1. The average recoveries were between 86. 2% -115.9%, RSD was between 1.7%-4. 7%. Conclusion： of the three extraction methods used, n - hexane - dichloromethane - light petroleum extracted in turn gave the best results. With its accuracy, high sensitivity, reproducibilitv .the method was feasible for quality control for health products.

关 键 词：毛细管气相色谱法 六六六 滴滴涕 保健品 

分 类 号：R917[医药卫生—药物分析学]