番茄雄性不育系HL-2快速繁殖的初步研究  

PRELIMINARY STDUDY ON THE RAPID PROPAGATION METHOD OF MALE STERILITY OF TOMATO

作　　者：毛秀杰[1] 闫见敏[2] 班淑菊 陈秀丽[4] 闫长见 

机构地区：[1]河北科技师范学院园艺园林系,秦皇岛066600 [2]西北农林科技大学园艺学院,杨凌712100 [3]吉林省梅河口市农业技术推广总站,梅河口135000 [4]吉林省榆树市农业技术推广中心,榆树130000 [5]河北省广平县职教中心,广平057650

出　　处：《内蒙古农业大学学报（自然科学版）》2007年第3期235-238,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：河北省科技厅"日光温室专用鲜食番茄品种选育"(05220182);河北科技师范学院博士基金资助

摘　　要：以番茄雄性不育系HL-2的叶片、带芽茎段为外植体进行组织培养,研究了不同激素及其不同浓度组合对番茄雄性不育系愈伤组织诱导、不定芽分化的效果。结果表明,叶片较易形成愈伤组织,试验筛选出叶片进行培养时各阶段的培养基为MS+6-BA1.5mg/L^3.0mg/L+IAA0.1mg/L^0.5mg/L+30g/L蔗糖均可诱导出愈伤组织,但以MS+6-BA1.5mg/L+IAA0.1mg/L+30g/L蔗糖最佳;愈伤组织芽的分化以MS+6-BA2.0mg/L+IAA0.1mg/L+30g/L蔗糖最佳。The leaves and stem segments with bud of tomato of male sterility HL - 2 as the explant were cultured in tubes. The effect of different media with different hormone and their concentration on induction of callus, differentiation of adventitious bud were studied. The result showed that： Lamina were easy to form callus. Culture medium for explant as lamina were screen out through examination. Those culture medium included （ 1 ） ） MS ＋ 6 - BA1.5mg/L - 3.0mg/L ＋ IAA0. lmg/L - 0.5 mg/L ＋ 30g/1 sugar. They all could induced callus in MS Medium, but the most appropriate median culture stages were MS ＋ 6 - BA1.5mg/L ＋ IAA0.1 mg/L ＋ 30g,/L sugar for the formation of callus from the Leaves ; （2） MS ＋ 6 - BA2.0mg/L ＋ IAA1.0mg/L ＋ 30g/L sugar were best for the differentiation of buds, that were becoming the important base of the high - frequency transformation of tomato male sterility.

关 键 词：番茄 雄性不育系 愈伤组织 快速繁殖 

分 类 号：S641.203.8[农业科学—蔬菜学]