糖尿病大鼠脾脏核因子κB和肿瘤坏死因子-α表达及其相关性  

Expressions of TNF-α and NF-κB and their relation in diabetic rat spleen

作　　者：陈瑛[1] 张康兰[2] 丁吉元[3] 

机构地区：[1]济南军区青岛第一疗养院特检科,山东青岛266071 [2]济南军区总医院眼科,山东济南250031 [3]济南军区总医院病理科,山东济南250031

出　　处：《实用医药杂志》2007年第8期971-972,共2页Practical Journal of Medicine & Pharmacy

摘　　要：目的观察糖尿病大鼠脾脏的病理改变,检测核因子-κBp65、肿瘤坏死因子-α表达的变化,并探讨两者关系。方法采用右下腹腔内一次性注射链脲佐菌素60mg/kg诱导Wistar大鼠糖尿病模型26只,分别于成模后1、3、6个月观察脾组织的病理改变,采用免疫组化方法检测核因子-κBp65、肿瘤坏死因子-α表达变化。结果随糖尿病病程延长,脾组织发生病理改变,核因子-κBp65、肿瘤坏死因子-α表达显著增强,两者呈正相关。结论高糖环境下,核因子-κBp65、肿瘤坏死因子-α在大鼠脾组织显著表达,与糖尿病脾脏病理、功能改变相关。Objective To observe the changes of pathology and the expression of tumour necrosis factor-α （rNF-α） and nuclear factor-κB （NF-κB） in diabetic rat spleen.Methods A total of 26 streptozotocins- induced diabetic rats were included in this study, immunohistochemistry detection of TNF-α and NF-κB were taken at the Ist,3rd,6th month after establishment of nlodel respectively. Results The expressions of TNF-α and NF-KB were found in diabetic rat spleen and increased along with the development of diabetes.Conclusion The expression of TNF-α and NF-κB could be related with complication of spleen （pathological and functional changes） in the diabetic rats.

关 键 词：糖尿病 核因子-ΚBP65 肿瘤坏死因子-Α 免疫组织化学 

分 类 号：R587.1[医药卫生—内分泌] R392.11[医药卫生—内科学]