83 K光系统Ⅱ核心复合物不同激发的荧光光谱学  

Fluorescence Spectroscopy of Photosystem Ⅱ Core Complex with Different Excitation Wavelengths at 83K

作　　者：蔡霞[1] 王水才[2] 贺俊芳[2] 彭菊芳[3] 刘晓[1] 匡廷云[4] 

机构地区：[1]西北大学生命科学学院,西安710069 [2]中国科学院西安光学精密机械研究所瞬态光学技术国家重点实验室,西安710068 [3]陕西师范大学生命科学学院,西安710061 [4]中国科学院植物研究所光合作用研究中心,北京100093

出　　处：《光子学报》2007年第6期1128-1132,共5页Acta Photonica Sinica

基　　金：国家重点基础研究发展规划(G1998010100)资助

摘　　要：在83K低温下,利用稳态荧光光谱技术对光系统Ⅱ(PSⅡ)核心复合物中激发能的传递进行了研究,激励波长分别选择为436nm,480nm,495nm和507nm,得到4种波长激发下的稳态荧光光谱.经过比较发现其最大峰值所在的位置没有因激发波长的不同而发生改变,都在696nm处,在不同激发波长下经过高斯解析获得不同的谱带.根据发射光谱与吸收光谱的对应性,反映了不同的光谱特性,说明在不同波长光的激发下,核心复合物中能量传递的途径不同.同时,可以分析出在核心复合物中,至少有Chla667700.4,Chla668834.7,685.1,Chla668879.0,Chla669900.9,693.4,695.2,698.04种Chla组分参与了能量的传递.At 83 K,fluorescence spectroscopy of the core complex from Photosystem Ⅱ was studied by the method of steady state fluorescence spectroscopy. The excitation wavelengths were 436 nm, 480 nm, 495 nm and 507 nm respectively. The peaks of the four spectra upon different excitation wavelengths are at about 696nm. By Gaussian deconvolution analysis, different spectral bands were analyzed in spectra with different excitation wavelengths,which reflects they have different spectral properties. The way of energy transfer in the core complex of PS II is different when it is excited at different wavelengths. According to the relationship between the emission spectrum and the absorption spectrum, there are at least Chl a670^670.4 , Chl a483^684.7.685.1 ,Ch1 a687^689.0 and Ch1 a^690^690.9.6934.695.2.698.0 involved in the energy transfer in the core complex of PS Ⅱ.

关 键 词：光生物学 荧光光谱学 PSⅡ核心复合物 稳态荧光光谱 

分 类 号：Q631[生物学—生物物理学]