基于磁性纳米粒子固定技术的漆酶传感器用于垃圾堆肥中邻苯二酚的检测  

Catechol Biosensor Based on Immobilizing Laccase to Modified Core-shell Magnetic Nanoparticles Supported on Carbon Paste Electrode

作　　者：章毅[1] 曾光明[1] 汤琳[1] 郁红艳[1] 李建兵 

机构地区：[1]湖南大学环境科学与工程学院,长沙410082 [2]Environmental Engineering Program,University of Northern British Columbia,Prince George,British Columbia,V2N4Z9,Canada

出　　处：《环境科学》2007年第10期2320-2325,共6页Environmental Science

基　　金：高等学校博士学科点专项科研基金项目(20020532017);国家高技术研究发展计划(863)项目(2004AA649370);国家重点基础研究发展规划(973)项目(2005CB724203);高等学校优秀青年教师教学科研奖励计划项目

摘　　要：以邻苯二酚为检测目标,研究了一种基于核/壳磁性纳米粒子固定漆酶的邻苯二酚生物传感器制备方法及其在城市生活垃圾堆肥中的应用.制备Fe3O4磁性纳米颗粒,使用正硅酸乙酯（TEOS）和氨丙基三甲氧基硅烷（APTMS）将其功能化并利用戊二醛将漆酶共价固定,借助磁场吸附在磁性碳糊电极上,检测邻苯二酚.固定在电极表面的漆酶保持了很好的活性.该生物传感器的检测线性范围为7.5×107～2.75×10-4mol/L,检测下限达到7.5×10^-7 mol/L,达到稳态电流95%所需时间大概为70 s.将该传感器检测堆肥浸出液中酚的含量的结果与高效液相色谱法对比,两者非常接近.A catechol blosensor was developed and used to analyze compost extracts based on the immobilization of laccase on the surface of modified magnetic core-shall （Fe3O4-SiO2） nanoparticles. Laccase was convalently immobilized on the magnetic nanoparticles by glutaraldehyde, which were modified with amino groups on its surface. The resulting magnetic bio-nanoparticles were attached to the surface of carbon paste electrode with the help of a permanent magnet to determine catechol. The immobilization matrix provided a good microenvironment for retaining the bioactivity of laccase. The linear range for catechol determination was 7.5 × 10^-7 ～ 2.75 x 10-4 mol/L, with a detection limit of 7.5 × 10^-7 mol/L. The detection current reached 95% of the steady-state current within about 70 s. Catechol concentration in compost extracts were determined by laccase biosensor and HPLC, with approximately the same result.

关 键 词：Fe3O4磁性纳米颗粒 漆酶 邻苯二酚 堆肥 

分 类 号：X830.2[环境科学与工程—环境工程]