大鼠肢体缺血再灌注致肺损伤及高渗盐水的肺保护作用  

Rat lung injury following limbs ischemia reperfusion and the lung protective effect of hypertonic saline

作　　者：韩洪伟[1] 刘红[2] 海克蓉[2] 马聚峰[2] 

机构地区：[1]江苏省沭阳县人民医院 [2]宁夏医学院附属医院麻醉科,银川750004

出　　处：《陕西医学杂志》2007年第9期1125-1127,1168,共4页Shaanxi Medical Journal

基　　金：宁夏医学院大学生科技创新基金项目(No200507)

摘　　要：目的:观察大鼠肢体缺血再灌注(LIR)后肺损伤的发生以及静脉输注高渗盐水(HS)的肺保护作用。方法:将SD大鼠24只随机分为3组:正常对照组(C组)、模型对照组(M组)、模型+高渗盐水处理组(H组),分别行肺的病理形态学观察;批量检测肺组织湿重/干重比值(W/D)、肿瘤坏死因子-α(TNFα-)含量、髓过氧化物酶(MPO)活性、丙二醛(MDA)含量。结果:M组出现肺间质水肿及炎细胞浸润等病理改变,W/D、TNF-α、MPO和MDA均明显高于其他两组(P<0.05),而H组与C组间比较无显著性差异(P>0.05)。结论:大鼠肢体缺血再灌注后发生了肺损伤,静脉输注高渗盐水有一定的肺保护作用。Obiective： To observe lung injury following limbs ischemia reperfusion （LIR） and the lung protective effect of intravenous infusion of hypertonic saline（HS）. Methods： 24 Sprague-Dawley rats were randomly divided into 3 groups： normal control group （C group）, LIR model group（M group）, and LIR ＋ HS treatment group（H group）. Pathologic changes of pulmonary tissue were observed . Lung tissue wet mass/dry mass weight ratio （ W/D ）, tumor necrosis factor-α （ TNF-α ） contents, myeloperoxidase（MPO） activities, malondialdehyde（MDA） contents were measured respectively. Results M group had such pathologic changes as pulmonary interstitial edema and leucocytes infiltration. W/ID TNF-α,MPO and MDA increased significantly in M group compared with those in C and H groups, but the changes between H group and C groups were insignificant. Conclusion ： Lung injury occurred in rats after LIR and intravenous infusion of HS has some lung protective effects.

关 键 词：再灌注损伤/并发症 肺/损伤 盐水 高渗/治疗作用 模型 动物 大鼠 

分 类 号：R658[医药卫生—外科学]