反相高效液相色谱法测定胃炎片中原儿茶醛的含量  

Determination of protocatechuic aldehyde in Weiyan tablets by RP-HPLC

作　　者：王海龙[1] 王宝全[1] 赵庆华[1] 

机构地区：[1]济南军区联勤部药品仪器检验所,山东济南250022

出　　处：《实用医药杂志》2007年第5期574-575,共2页Practical Journal of Medicine & Pharmacy

摘　　要：目的建立胃炎片中原儿茶醛的含量测定方法。方法采用反相高效液相色谱法测定胃炎片中原儿茶醛的含量。采用日本Sil C18色谱柱,以甲醇-1%醋酸水溶液(13∶87)为流动相,流速为1ml/min,检测波长280nm。结果原儿茶醛在0．0762～0．4572μg线性关系良好,r=0．9999,平均回收率为101．1%, RSD=0．70%。结论该方法简便、灵敏、准确,可作为胃炎片的质量控制方法。Objective To establish a method for the determination of protocatechuic aldehyde in Weiyan tablels. Methods RP-HPLC method was developed for The delermination of protocatechuic aldehyde in Weiyan tablets on Japanese Sil C18 column, wilh nlethanol-l% acetic acid （13：87） as a mobile phase（lmL/min）and detection wavelength at 280 nm. Results The linear range of protocatechuic aldehyde was 0.0762-0.4572t,tg and Ihe correlation coefficient was 0.9999. The average recovery rate was 101,1%, RSI） was 0.70% （n=6）. Conclusion The method is convenient and accurate, and can be used control the tablets quality.

关 键 词：高效液相色谱 胃炎片 原儿茶醛 

分 类 号：R927.2[医药卫生—药学]