水苏糖对模拟异种心脏移植超急性排斥反应的抑制作用  

Inhibitory effects of stachyose on hyperacute rejection in pig-to-human heart xenotransplantation

作　　者：刘波[1] 倪科伟[2] 周冰[2] 许林海[2] 詹金彪[1] 

机构地区：[1]浙江大学医学院生物化学与遗传学系,杭州310058 [2]浙江省人民医院心胸外科

出　　处：《中华器官移植杂志》2007年第10期582-584,共3页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金(30470369)

摘　　要：目的观察水苏糖对猪到人异种心脏移植超急性排斥反应的抑制作用。方法以人血液体外灌注猪游离心脏为基础,模拟猪到人异种心脏移植的超急性排斥反应模型。实验分为A、B两组:即分别用人血液灌注及人血液加水苏糖灌注猪离体心脏。观察灌注后两组心脏的跳动时间;体外灌注1 h后,对两组灌注心脏进行免疫组织化学(测定IgG及IgM的沉积)及病理学分析。结果A组灌注心脏平均跳动时间为(9.5±2.5)min B组灌注心脏平均跳动时间为(46.8±8.1)min,其中有1个心脏在灌注的1 h内一直跳动;两组心脏跳动时间比较,差异有统计学意义(P<0.01)。A组心肌间质旱弥漫性出血、水肿,血管扩张,内皮细胞肿胀、坏死;免疫组织化学检查显示心肌血管内皮组织中有IgG及IgM沉积。B组心肌间质未见出、凝血和坏死,血管内皮细胞未见肿胀;免疫组织化学检测未见IgG及IgM沉积。结论水苏糖对异种心脏移植超急性排斥反应具有抑制作用。Objective To study the inhibitory effects of stachyose on the hyperacute rejection in pig-to-human heart xenotransplantation. Methods A pig-to-human xenogeneic heart transplantation model was established based on an in vitro free heart blood perfusion system. The pig hearts were divided into two groups： group A （pig hearts treated with human blood perfusion as control） and group B （pig hearts treated with human blood plus stachyose perfusion）. After perfusion for I h, the heart xenografts were examined for deposit of IgG and IgM by immunohistochemistry and pathological analy sis. Results The mean survival time of perfusion hearts in groups A and B was （9. 5 ± 2. 5） min and （46. 8 ± 8. 1 ） min respectively （P〈0. 01 ）. Histopathological studies revealed that haemorrhagia, edema, necrosis of endothelial cells, distension of blood vessel were found in group A, but in group B no obvious pathological changes were found. Immunohistochemistry studies also indicated there was obvious deposit of IgG and IgM in group A while in group B no deposit of IgG or IgM was found. Conclusion Stachyose can inhibit the hyperacute rejection in pig-human xenotransplantation.

关 键 词：移植物排斥 水苏糖 移植 异种 猪 

分 类 号：R654.2[医药卫生—外科学]