结扎切断法与白芨微粒栓塞法建立大鼠后肢缺血模型效果比较  

A comparison of a rat hind-limb ischemia model established by ligation and embolization

作　　者：梁翠宏[1] 田铧[2] 徐蕴[2] 李贵宝[2] 田广平[2] 宋涛[2] 

机构地区：[1]潍坊医学院解剖教研室,山东潍坊261042 [2]山东大学医学院解剖学教研室,济南250012

出　　处：《山东大学学报（医学版）》2007年第10期1008-1010,1015,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Y2005C14)

摘　　要：目的采用结扎法和白芨微粒栓塞法制作大鼠后肢缺血模型，并对其缺血效果加以比较，以探寻有效的建模方法。方法取Wistar大鼠分别用结扎股动脉法、结扎髂总动脉法、白芨微粒栓塞法建立大鼠后肢缺血模型。术后1～21d观察大鼠后肢缺血情况及功能改变，取材作病理切片观察。结杲①结扎股动脉或髂总动脉后大鼠后肢仅出现短暂的跛行，组织切片未见异常改变；②白芨微粒栓塞股动脉后，大鼠后肢出现持续跛行、肌肉萎缩和皮肤坏死等表现，组织切片显示大鼠后肢皮肤、肌肉组织均出现明显而持久的缺血或坏死。结论①结扎血管法不能造成明显的大鼠后肢缺血；②白芨微粒栓塞股动脉法可引起大鼠后肢明显的持续性缺血，是一种较为理想的大鼠后肢缺血模型制作方法。Objective To explore an effective method for establishing a hind-limb ischemia model in rats. Meth. otis Wistar rat hind-limb ischemia models were established by three methods： hgation of the femoral artery, ligation of the common iliac artery, embolization of the femoral artery with fine particles of the common bletilla tuber （FP-CBT）. For 1 to 21 days after operation, the ischemia and function were observed. Results ① Temporal limp happened in the operated hind-limbs after ligafing the femoral artery or the common iliac artery, and no pathological changes were found in the tissue sections; ② At 3 to 21 days after embolizing the femoral artery by FP-CBT, severe ischemia occurred in the operated hind-limbs, such as that sustained by limping, skin necrosis or ulcers, and muscle fibers necrosis which was recognized by HE staining. Conclusion Hindlimb ischemia can not be produced by ligating the artery. However, it can be well induced by embolizing the femoral artery with FP-CBT.

关 键 词：缺血模型 结扎法 白芨 栓塞 后肢 大鼠 

分 类 号：R332[医药卫生—人体生理学]