微阵列检测血样感染相关四项指标的实验研究  

Protein microarray for simultaneous detection of pathogens in blood transfusion

作　　者：黄敏[1] 张敏丽[2] 蒲晓允[1] 

机构地区：[1]第三军医大学新桥医院检验科,重庆400037 [2]军事医学科学院放射医学研究所,北京100871

出　　处：《第三军医大学学报》2007年第21期2080-2082,共3页Journal of Third Military Medical University

摘　　要：目的利用蛋白芯片技术检测血样感染相关四项指标,并与ELISA酶联免疫技术进行客观分析评价。方法对230份国家参比品同时进行蛋白芯片与ELISA酶联免疫的检测,并观察蛋白芯片对诊断血样感染四项指标的敏感性和特异性。结果用统计学的配对计数资料检验得出:χ2=0.5,P=0.125,说明两种方法检测结果无统计学差异。结论蛋白微阵列检测结果无论是与参比品本身还是与ELISA试剂盒都有极高的符合率,是一种很有发展前途的检验技术。Objective To study the diagnostic value of multiplex protein microarray and ELISA for human serum pathogens. Methods Totally 230 national standard samples were tested by multiplex protein microarray and ELISA respectively for the pathogens of HBV, HIV, HCV and treponema pallidum （TP）. Results The matched pair test for numeration data showed χ^2 = 0.5, P = 0. 125, indicating that no statistical significance between the two methods. Conclusion Multiplex protein microarray shows high specificity and sensitivity to the four pathogens so that it is worth extensive application.

关 键 词：蛋白微阵列 乙型肝炎病毒 人类免疫缺陷病毒 丙型肝炎病毒 梅毒螺旋体 

分 类 号：R319[医药卫生—基础医学] R446.11