EGF和不同血清对牛卵母细胞成熟及核移植胚体外发育的影响  

Effect of EGF and different serum on the maturation of bovine oocytes and the development of bovinesomatic nuclear transfer embryos in vitro

作　　者：王亚刚[1] 隋丽娜[1] 彭新荣[1] 赛务加甫[1] 郭志林[1] 李向臣[1] 王勇胜[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2007年第10期25-28,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"计划项目(2004AA213072)

摘　　要：分离牛卵母细胞,分别用添加表皮生长因子(EGF)和发情牛血清(发情当天血清(OCS(0 d))和发情第7天血清(OCS(7 d)))的培养液进行成熟培养后去核,构建牛体细胞核移植胚并进行体外培养,研究EGF和OCS对卵母细胞成熟及核移植胚体外发育的影响。结果表明EGF添加组和OCS(0 d)添加组卵细胞的成熟率及核移植胚的卵裂率和囊胚率分别为74.5%,84.6%,25.9%和83.9%,78.8%,31.3%。在牛卵母细胞成熟液中添加EGF、OCS(0 d)更有利于牛核移植胚胎的发育。Oocytes were isolated from bovine ovaries,enucleated after maturation in maturation medium with EGF or OCS （0 d and 7 d） ,respectively. Nuclear transfer embryos were constructed and cultured in vitro, and the influence of EGF and OCS on oocyte maturation was also studied. The results showed that the rates of maturation,cleavage and blastoaysts on group adding EGF and group adding OCS（0 d） were 74.5% ,84. 6%, 25. 9% and 83. 4%, 78. 7%, 31. 3%, respectively. The results indicate that the nuclear transfer embryos＇ development is better in the oocytes maturation medium with EGF or OSC（0d）.

关 键 词：表皮生长因子 发情牛血清 胎牛血清 核移植胚 

分 类 号：S823[农业科学—畜牧学] Q813.6[农业科学—畜牧兽医]