猪SOCS-2基因cDNA全长序列的克隆与组织表达  

Full cDNA cloning and tissue distribution of SOCS-2 gene in pig

作　　者：杜宝文[1] 杨公社[1] 孙超[1] 

机构地区：[1]西北农林科技大学动物脂肪沉积与肌肉发育实验室,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2007年第10期39-43,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(NO30471267);教育部重点项目(NO105167);校青年学术骨干支持计划项目

摘　　要：根据Genbank已登录的细胞因子信号转导抑制因子(SOCS-2)基因序列同源区设计1对引物,从8月龄健康雄性八眉猪肾脏组织中提取总RNA,利用RT-PCR技术扩增猪SOCS-2基因;用CLUSTAL W软件进行同源性分析,利用半定量(Semi-quantitative,SQ)RT-PCR技术检测八眉猪各组织中SOCS-2 mRNA的表达量。结果表明,猪SOCS-2基因cDNA全长克隆成功(Genbank登录号为EF121242);家猪SOCS-2基因与澳洲野猪、人、小鼠、大鼠SOCS-2核苷酸同源性分别为99%,94%,91%,89%,SOCS-2蛋白氨基酸序列同源性分别达到100%,95%,94%,93%;SOCS-2基因在心脏、肺、皮下脂肪组织、腹部脂肪组织、肝脏、脾、肾脏和肌肉组织中广泛表达,其中肾脏组织中最高,心脏次之,肌肉组织中表达量最低。表明猪SOCS-2序列保守性较高,在体内可能具有多种功能。SOCS-2 was very important to growth and development of organism and growth hormone signaling. A pair of primers were designed according to the homology region of SOCS-2 among other species. Total RNA was isolated from kidney of Bamei pig,a local strain of Chinese pig,and the complete cD- NA sequence of SOCS-2 gene was cloned by RT-PCR technology firstly（Genbank accepted number is EF121242）. Thereafter, the distribution of SOCS-2 mRNA in Bamei pig was analyzed by SQ-RT PCR. Compared with others,the homology of SOCS-2 to Sus scrofa,human,mouse and rat was 99%,94%,91% and 89 %, respectively, but the homology of protein reached 100 %, 95 %, 94% and 93 %, respectively. And the data showed that the SOCS-2 could be detected in many tissues extensively,such as heart,lung,subcutaneous adipose,abdominal adipose,liver,spleen,kidney and muscle. Its expression was higher in kidney and heart but lower in muscle tissue.

关 键 词：SOCS2 猪 SQ RT—PCR 组织表达 

分 类 号：Q786[生物学—分子生物学]