苹果叶片抗坏血酸过氧化物酶基因cDNA的克隆  

Cloning of ascorbate peroxidase cDNA from apple

作　　者：粟霞[1] 张军科[1] 马锋旺[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2007年第10期77-80,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省重大科技专项计划项目(2006kz05-G4);西北农林科技大学"拔尖人才支持计划"项目

摘　　要：根据Genbank上已报道的烟草、辣椒、龙眼和杨树4种植物的抗坏血酸过氧化物酶基因的保守区域设计兼并引物,利用RT-PCR和RACE法对皇家嘎拉苹果叶片APX基因进行克隆、分析。结果表明,皇家嘎拉苹果叶片APX基因片段长度为694 bp,与GenBank中已登陆的富士苹果果实、皇家嘎拉苹果花芽、草莓及Rosa hybridcultivar等的APX cDNA的同源性分别为97%,97%,84%和84%。With degenerated primers designed according to conservative region of ascorbate peroxidase gene sequence of Nicotiana tabacum, Capsicum annuum, Dimocarpus longan, and Populus curamericana on Genbank,the fragment of APX gene was cloned from Royal Gala apple by RT-PCR method and the 3＇ end by rapid amplification of cDNA end （RACE） strategy. The analysis showed that the spliced fragment contained 694 base pairs,which were 97%,97%,84% and 84% homology to that of Fuji fruit,Royal Gala young shoot, strawberry and Rosa hybrid cultivar in APX eDNA of Genbank,respectively.

关 键 词：苹果叶片 APX基因 过氧化物酶 

分 类 号：S661.103.2[农业科学—果树学]