硫酸软骨素酶ABC对大鼠脊髓损伤后硫酸软骨素蛋白多糖与生长相关蛋白43表达的影响  

Effects of chondroitinase ABC on chondroitin sulfate proteoglycans and growth associated protein 43 after spinal cord injury in rat

作　　者：林扬元[1] 谢群[1] 陈建明[1] 王玮[2] 

机构地区：[1]莆田学院医学院人体解剖与组织胚胎学教研室,莆田351100 [2]福建医科大学人体解剖学与组织胚胎学系,福州350001

出　　处：《解剖学杂志》2007年第5期582-584,601,共4页Chinese Journal of Anatomy

基　　金：福建省教育厅科技项目(JA05322)

摘　　要：目的:探讨硫酸软骨素酶ABC对严重的大鼠脊髓损伤蛋白多糖及生长相关蛋白43(GAP-43)基因表达的影响。方法:制作大鼠脊髓横断伤模型,分为脊髓损伤组和脊髓损伤治疗组,给于治疗组硫酸软骨素酶ABC鞘内注射,用免疫荧光组织化学观察硫酸软骨素蛋白多糖(CSPGs)的表达,半定量RT-PCR、Western印迹法观察GAP-43 mR- NA及蛋白的表达。结果:治疗组与损伤组比较,CSPGs的表达减少,差异具有显著性,而GAP-43mRNA及蛋白表达增多。结论:硫酸软骨素酶ABC能够裂解CSPGs,改善脊髓损伤区的微环境,促进GAP-43 mRNA及蛋白的表达,是修复脊髓损伤和促进神经再生的机制之一。Objective： To investigate the effects of chondroitinase ABC on chondroitin sulfate proteoglycans （CSPGs） and growth associated protein-43 （GAP-43） after spinal cord injury in rats. Methods： The model of rat spinal cord transection was made and divided into spinal cord injury group （Group A） and treatment group （Group B）, which was injected with 20/A chondroitinase ABC （0.2 units/ml）. Immunofluorescent histochemical staining was used to observe the dissolving effect of chondroitin sulfate proteoglycans; Then GAP-43 was tested by semiquantitative RT-PCR and Western-blot. Results： The fluorescence intensity of CSPGs of Group B was significantly less than that of group A （P〈0. 05）. Contrarily the expressions of GAP-43 mRNA and protein in Group B were higher than those in Group A （P〈0. 05）. Conclusion： Chondroitinase ABC might split CSPGs, improve microenvironment of the injured region,and enhance the expression of GAP-43, which is one of the mechanism of repairing the spinal cord injury.

关 键 词：硫酸软骨素酶ABC 脊髓损伤 硫酸软骨素蛋白多糖 生长相关蛋白43 

分 类 号：R651.2[医药卫生—外科学]