丹参对实验性大鼠溃疡性结肠炎预防机制研究  

Protective effect of ulcerative colitis with Tanshinon in rats

作　　者：刘建生[1] 田怡[1] 张晓红[1] 祝子华[1] 孙菁[2] 袁耀宗[2] 吴云林[2] 

机构地区：[1]上海瑞金医院集团闵行区中心医院消化科,上海201100 [2]上海交通大学医学院附属瑞金医院消化科

出　　处：《中国医师杂志》2007年第10期1324-1326,共3页Journal of Chinese Physician

基　　金：上海市科学技术委员会科研计划资助项目（044119751）

摘　　要：目的研究丹参对大鼠溃疡性结肠炎（UC）预防机制。方法予雌性Sprague-Dawley大鼠含30 mg三硝基苯磺酸（TNBS）的50%乙醇溶液0.85 ml灌肠,诱导实验性溃疡性结肠炎。丹参预防组,造模前先用丹参从尾静脉注入丹参注射液2 ml.kg-1.d-1连续3 d。造模7 d后,正常对照组、丹参预防组和造模组尾静脉注入0.9%NaCl溶液1 ml/d;丹参治疗组每天尾静脉注入丹参注射液2 ml.kg-1.d-1。通过肠道重量指数、溃疡面积、大体形态和组织学评分及血和肠黏膜中一氧化氮（NO）、超氧化歧化酶（SOD）含量测定评估疗效。结果丹参预防组肠道重量指数、溃疡面积、大体形态和组织学评分及血和肠黏膜中NO含量减少、SOD含量增高,与丹参治疗组、模型组比较差异有统计学意义（P〈0.01）;但较正常对照组NO含量仍增高和SOD含量减少。结论丹参有效预防大鼠UC发生和发展,可能丹参具有拮抗NO、氧自由基机制有关。Objective The protective effect of ulcerative colitis with Tanshinon in rats. Methods 30 mg of trinitro-benzene-sulfonic acid （TNBS） dissolved in 0, 85 ml of 50% ethanol was administrated intrarectally in Sprague-Dawley female rats to induce experimental colitis. One pretreated intravenously with Tanshinon 2 ml · kg^-1·d^-1 were 3 days. After 7 days, the rats were divided with normal control group, 0.9% saline group, protective group （0.9% NaCl 1ml/d） and treatment group （Tanshinon 2 ml·kg^-1·d^-1 ）, The therapeutic effects were evaluated by measuring the ponderal index, the surface area of the ulcers, macroscopical and histological score, activity of NO and SOD in colonic tissue and serum in all rats. Results Compared with the treatment in Tanshinon and saline group, the ponderal index, surface area of the ulcers, macroscopical and histological score, activity of NO level in the serum and intestine mucosal decreased, and SOD significantly increased in the pretreated group. The level of NO significantly increased and SOD decreased in the pretreated group compared with the normal control group. Conclusion Tanshinon is effective in the treatment of UC by antagonizing of NO and SOD in rats.

关 键 词：丹参△/药理学 结肠炎 溃疡性/中药疗法 

分 类 号：R28[医药卫生—中药学]