细极链格孢菌蛋白激发子对棉主要性状及相关酶的影响  

Effect of protein elicitor from Alternaria tenuissima on main characters and enzymatic activity of cotton

作　　者：张志刚[1] 邱德文[2] 曾洪梅[2] 杨秀芬[2] 官春云[1] 梅正鼎[3] 杨晓萍[3] 

机构地区：[1]湖南农业大学农学院,长沙410128 [2]中国农业科学院植物保护研究所,北京100081 [3]湖南省棉花科学研究所,湖南常德415101

出　　处：《中国农业大学学报》2007年第5期16-21,共6页Journal of China Agricultural University

基　　金：国家973资助项目(2003CB114204);国家863资助项目(2003AA241130)

摘　　要：为了给细极链格孢菌蛋白激发子的田间使用提供依据,测定了其粗提蛋白液对棉主要性状及相关酶活性变化影响的相关数据。结果表明:细极链格孢菌蛋白激发子诱导后显著提高了棉成铃数,且不同程度提高了株高、单铃重、衣指、子指和衣分,降低了单铃壳重,特别是促进了棉花早熟,获得了较高的产量。以浸种处理的籽/皮棉产量最高,分别达到3 800.60和1 598.15 kg/hm2,比对照提高了22.15%和26.11%;纤维的主要品质性状指标与对照间差异均达到了极显著水平,浸种+现蕾期喷雾处理的综合值比对照提高6.1%,为最高;诱导后POD(过氧化物酶)、SOD(超氧化物歧化酶)及NR(硝酸还原酶)的酶比活性增强,其中POD酶比活性均值最高为(502.00±1.10)μmol/(g.min),SOD为(378.36±0.43)μmol/(mg.min),NR为(168.96±2.30)μg/(g.h);而MDA(丙二醛)含量降低,最低为(2.814±0.090 9)μmol/g。The effect of protein elicitor from Alternaria tenuissima on main characteristics and enzymatic activity of cotton. Was studied. The number of plant bell was significantly promoted by Alternaria tenuissima protein elicitor. The plant height, weight of single bell, lint-index, seed-index and lint-percent were increased in different degrees, whereas the weight of single bell hull was decreased. The protein elicitor promoted the growth and yield of cotton. Seed treatment by soaking seeds increased the unginned and lint-yield to 3 800.60 and 1 596.15 kg/hm^2, respectively, being 22.15% and 26.11% higher than those of control. Many parameters were markedly improved by applying the protein activator at the right time and concentration. The Seed soaking plus spraying at the bud development stage was the most efficient. POD（peroxidase）, NR（nitrate reductase） and SOD（superoxide dismulasc） were increased in all treatments by Alternaria tenuissima protein elicitor. MDA （malondialdehyde） decreased in all treatments studied.

关 键 词：链格孢菌 蛋白激发子 棉 产量 MDA SOD POD NR 

分 类 号：S562.035.3[农业科学—作物学]