中成药甘舒胶囊对抗氧化应激诱导的肝细胞损伤  

Ganshu capsule protects Chang liver cells against damage induced by oxidative stress

作　　者：叶锦辉[1] 曾颖[2] 职君利[3] 严群超[4] 赵春梅[5] 崔宇[5] 郭瑞鲜[5] 姚巧玲[5] 冯鉴强[5] 廖新学[6] 

机构地区：[1]广东省人民医院东病区,广东广州510080 [2]广州市番禺区中医院 [3]广东药学院 [4]暨南大学医学院内科 [5]中山大学中山医学院生理学教研室 [6]中山大学附属第一医院心血管内科

出　　处：《解剖学研究》2007年第5期349-353,共5页Anatomy Research

基　　金：广州市番禺区卫生局2005年科研基金资助

摘　　要：目的探讨中药甘舒胶囊对抗氧化应激损伤的肝细胞保护作用。方法在Chang肝细胞建立氧化应激(H2O2)损伤的实验模型,应用甲氮甲唑蓝(MTT)检测法。PI染色流式细胞仪(FCM)及Hoechst 33258染色法等检测甘舒对抗H2O2诱导Chang肝细胞的细胞毒性及凋亡的细胞保护作用。结果H2O2呈浓度依赖性地降低Chang肝细胞的存活率;在自身不影响Chang肝细胞存活率的浓度(1～100μg/ml)范围内,甘舒呈浓度依赖性地对抗300μmol/L和400μmol/LH2O2对肝细胞存活率的抑制作用;另方面,在0～800μmol/L浓度范围内,H2O2呈浓度依赖性地增加Chang肝细胞的凋亡率;100μg/ml、500μg/ml和1mg/ml的甘舒本身不影响肝细胞的凋亡率,但却能显著地抑制300μmol/LH2O2诱导的肝细胞凋亡。结论中药甘舒胶囊具有抗氧化应激作用,可显著对抗氧化应激(H2O2)诱导的肝细胞损伤。Objective To explore the protective effect of Ganshu capsule （traditional Chinese medicine） on damage of Chang live cells induced by oxidative stress. Methods To establish the experiment model of oxidative stress （H2O2）-induced damage in Chang liver cells. The viability of Chang liver cells was evaluated by MTT assay. Apoptosis of Chang liver cells was detected by PI stain flow cytometer and Hoechst 33258 stain respectively. Results H2O2 reduced the viability of Chang liver cells dose-dependently. At the concentrations from 1 μg/ml to 100 μg/ml, which did not affect the viability of Chang liver cells, Ganshu capsule blocked dose-dependently the inhibition of H202 （300 μmol/L or 400 μmol/L） on the viability of Chang liver cells. Furthermore, at the concentrations from 0 μg/ml to 800 μmol/L, H202 induced apoptosis of Chang liver cells dosedependently. Ganshu capsule at 100 μg/ml, 500 μg/ml and 1 mg/ml which did not Chang the apoptotic percent of Chang liver cells inhibited apoptosis of the cells induced by 300 μmol/L H2O2 obviously. Conclusion Ganshu capsule may antagonize oxidative stress and protect Chang liver cells against damage induced by H2O2 significantly.

关 键 词：中药 甘舒胶囊 过氧化氢 肝细胞 凋亡 

分 类 号：R285[医药卫生—中药学]