应用RAPD标记对高粱属两物种之间遗传差异的研究(简报)  

A study on genetic variation of two species of sorghum by RAPD markers

作　　者：李杰勤[1] 王丽华[1] 詹秋文[1] 李云飞[1] 

机构地区：[1]安徽科技学院,安徽凤阳233100

出　　处：《草业学报》2007年第5期140-144,共5页Acta Prataculturae Sinica

基　　金：安徽科技学院引进人才项目(ZRC200564);安徽省科技攻关计划重点项目(06013104B)资助

摘　　要：本研究选用了32个栽培高粱品种、10个苏丹草品种及2个高粱近缘种进行了RAPD分析。结果表明,1)12对引物产生的68条DNA扩增片段中,52条(76.5%)具有多态性。2)高粱之间的相似系数从55%到95%;苏丹草之间的相似系数从52%到84%,因此选择的品种之间的多态性高,具有代表意义。高粱不育系和保持系之间的相似度很大,为89%以上,但通过RAPD标记能够将其区分。3)以0.66为阈值将44个品种分为10个类群。第1类群中全部为苏丹草;在2,3,4群中既有苏丹草也有高粱;5,6,7,8群中则全部为高粱。因此,使用RAPD分子标记进行聚类不能将高粱和苏丹草区分开来。Thirty two accessions of Sorghum bicolor, 10 varieties of Sorghum sudanense and two related species of S. bicolor were analyzed using random amplified polymorphic DNA（RAPD） markers. A total of 68 bands were amplified by 12 primers and 52 （76.5%） bands were found to be polymorphic. The similarity coefficient of S. bicolor and S. sudanense were 55% to 95% and 52% to 84% ,respectively. Polymorphy was high in both. Restorer and maintainer lines of S. bicolor could be differentiated by RAPD although the similarity was more than 89%. Cluster analysis showed that the 44 varieties fell into 10 groups using a threshold value of 0.66. The whole accession of the first group were S. sudanense and the whole accession of the 5, 6, 7 and 8 groups were S. bicolor. Accessions of S. sudanense and S. bicolor were in the number 2, 3 and 4 groups. It suggested that S. bicolor and S. sudanense cannot be distinguished by RAPD.

关 键 词：高粱 苏丹草 聚类分析 RAPD 

分 类 号：S514[农业科学—作物学]