重离子和X射线照射对小鼠骨髓细胞周期分布的影响  

Effects of heavy ions and X-ray irradiations on mice bone marrow cell cycle distribution

作　　者：邴涛[1] 胡相凯[1] 党秉荣[1] 谢漪[1] 荆西刚[1] 李文建[1] 

机构地区：[1]中国科学院近代物理研究所,兰州750000

出　　处：《中华放射医学与防护杂志》2007年第5期425-426,共2页Chinese Journal of Radiological Medicine and Protection

基　　金：“西部之光”资助项目（XB040602）;国家自然科学基金重点资助项目（10335050）;甘肃省青年科学基金资助项目（3ES041-A25-025）志谢感谢甘肃省医学科学院毒理与药理研究室的郭红云、李雪萍、李德杏、梁涛、高波、胡清荣和张永东等老师,以及放射科的王小虎主任医师

摘　　要：目的 研究重离子和X射线全身照射小鼠对骨髓细胞周期分布的影响，为面向生物学对象的重离子治疗提供基础数据。方法用X射线和重离子照射BALB／c小鼠后，24h后制得骨髓细胞，采用PI染色用流式细胞仪测骨髓细胞周期变化。结果照射24h后，X射线照射的各组骨髓细胞周期分布与对照组差异没有统计学意义；重离子照射组与对照组相比，出现G1／G0期和G1／M期阻滞，与相同剂量X射线照射组相比亦出现G1／G0期和G2／M期阻滞。结论 相同剂量的重离子照射较X射线对骨髓细胞的损伤更严重，引发不同的生物学效应。Objective To investigate the effects of whole body irradiation with heavy ions and X-ray at different doses on mice bone marrow cell cycle distribution, and to provide the basic data for biological-objectoriented hadrontherapy（OOBH）. Methods The BALB/c mice were irradiated with heavy ions and X-ray, and the bone marrow cells were harvested after 24 hours, and then the cell cycle distribution were analyzed by flow cytometry. Results At 24 h after irradiation, bone marrow cell cycle distribution of the X-ray groups were not different from the control. While in the heavy ions irradiation groups, G1/G0 phase and G2/M phase were markedly arrested compared with the control group as well as X-ray irradiation group at the same dose. Conclusions The heavy ions irradiation was more dangerous on the mice bone marrow cells than the X-ray irradiation at the same doses. They induced different relative biological effects.

关 键 词：重离子照射 X射线 细胞周期 骨髓细胞 

分 类 号：R686[医药卫生—骨科学]