电离辐射对巨核细胞与白介素3及其受体基因表达的影响  

Effects of irradiaiton on expression of IL-3 and IL-3Rα in Dami cells

作　　者：韩京[1] 朱才众[1] 冉新泽[1] 粟永萍[1] 郑怀恩[1] 程天民[1] 

机构地区：[1]第三军医大学预防医学院全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆400038

出　　处：《中华放射医学与防护杂志》2007年第5期430-432,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金重点资助项目（30230360）

摘　　要：目的 探讨辐射损伤对巨核细胞增殖和细胞周期的影响及其调控因子IL-3及其受体（IL-3Rα）表达的变化。方法 利用MTT法检测辐射损伤后Dami细胞的增殖作用，并通过流式细胞仪检测细胞周期的变化。用RT-PCR方法检测辐射后Dami细胞IL-3和IL-3Rα表达水平的变化。结果Dami细胞在受到不同剂量7射线照射后均发生增殖抑制，并发生明显的G0，G1期阻滞。辐射损伤5、10和15Gv后，IL-3和IL-3Rα在mRNA水平表达均显著低于对照组（P〈0．05），其变化趋势是随照射剂量增加而表达逐渐降低。结论不同剂量辐射所致造血功能障碍与巨核细胞增殖抑制、细胞周期改变和调控因子IL-3和IL-3Rα的异常表达有关。Objective To analyse the cell proliferation, cell cycle and the expression of IL-3, IL-3Rα in Dami cells after exposure to 60 Co y rays. Methods After Dami cells were exposed to 60 Co y rays, the cell cycle and proliferation of Dami cells were analyzed by means of MTT and flow cytometry. The IL-3 and the IL- 3Rα expressions were detected by RT-PCR at 24 h after irradiation. Results The irradiated Dami cells showed dose-dependent proliferative inhibition and G0/G1 block, Expressions of IL-3 and IL-3Rα mRNA in Dami after irradiation at doses of 5, 10, and 15 Gy were significantly lower than those in normal Dami cells （P 〈 0.05）, Their expressions showed reverse correlation with the dose. Conclusions y rays irradiation change the cell cycle, inhibits cell proliferation, IL-3 and IL-3Rα mRNA expression, suggesting that the abnormal expressions of IL-3 and IL-3Rα in Dami cells are involved in the injury of hemopoiesis by different doses of 60 Co γ ray irradiation.

关 键 词：辐射 巨核细胞 细胞周期 IL-3 IL-3Ra 

分 类 号：R686[医药卫生—骨科学]