阿托伐他汀对急性心梗大鼠心肌血管新生及eNOS及RhoA表达的影响  

Effects of Atorvastatin on Angiogenesis and Expression of eNOS and RhoA in Acute Myocardial Infarction Rats

作　　者：周卿[1] 陈书艳[1] 林强[1] 张琼[1] 颜雪芸[1] 王飞[1] 

机构地区：[1]上海交通大学医学院附属新华医院心内科,上海200092

出　　处：《中国微循环》2007年第5期289-292,349,共5页Journal of Chinese Microcirculation

摘　　要：目的观察不同剂量阿托伐他汀对急性心梗大鼠心肌血管新生及心肌eNOS及RhoA表达的影响。方法雄性急性心肌梗死大鼠60只,随机分为四组,即小剂量阿托伐他汀(3 mg.kg-1.d-1)组、中剂量阿托伐他汀(12 mg.kg-1.d-1)组、大剂量阿托伐他汀(50 mg.kg-1.d-1)组、对照组,每组15只。每只给予连续灌胃2周,对照组给予生理盐水。2周后处死动物,α-SMA及vWF免疫组化染色检测心肌血管密度;逆转录-聚合酶链反应法(RT-PCR)检测内皮型一氧化氮合酶(eNOS)及RhoA mRNA的表达。结果大剂量组α-SMA染色阳性血管计数较对照组明显减少,有极显著性差异(P<0.01);中剂量组vWF染色阳性微血管计数较对照组明显增加,有极显著差异(P<0.01)。中剂量组eNOS mRNA表达较对照组明显增加,有显著性差异(P<0.05)。大剂量组RhoA mRNA表达较对照组明显减少,有显著性差异(P<0.05)。结论阿托伐他汀对大鼠急性心肌梗死后心肌血管新生的作用与剂量有关。eNOS、RhoA可能参与阿托伐他汀对急性梗死心肌血管新生作用过程。Objective To investigate the effect of different dose of atorvastain on angiogenesis in AMI myocardium in rats, and its relationship between eNOS and RhoA expressed in myocardium. Methods Male Sprague-Dawley rats with AMI were randomly treated with normal saline（control）, atorvastatin 3mg/kg· d（ low dose group）, 12mg/kg· d（ medium dose group） and 50 mg/kg· d （ high dose group） by gavage for 2 weeks starting from the first day after MI. The rats were executed after being administered for 14 days. The microvessels and arterioles of myocardium were assessed by vWF and α-SMA immunohistochemistry. The eNOS, RhoA mRNA expression of myocardium were measured by reverse-transcriptase polymerase chain reaction （ RT- PCR）. Results Compared with control group, the number of α-SMA staining positive arterioles decreased in high dose group（ P 〈 0.05）. The number of vWF staining positive microvessels increased in medium dose group（P 〈0.01）. The eNOS mRNA expression in myocardium increased in medium dose group（ P 〈 0.05） compared with control group. The RhoA mRNA expression in myocardium decreased in high dose group（ P 〈 0. 05）, respectively. Conclusion The effects of atorvastatin on angiogenesis were related to the doses. The expression of eNOS and RhoA might participate in the course of angiogenesis.

关 键 词：血管新生 阿托伐他汀 ENOS RHOA 急性心肌梗死 

分 类 号：R542.22[医药卫生—心血管疾病] R332.12[医药卫生—内科学]