CD_(25)/IL-2Ra在人肝组织毛细胆管上的表达  

Expressions of CD_(25)/IL-2Ra on Cholangiole of Human Liver

作　　者：陆忠华[1] 印永强 裴浩[3] 黄利华[3] 邢益平[1] 

机构地区：[1]南京医科大学第一附属医院感染科,江苏南京210029 [2]无锡市妇幼保健医院病理科 [3]无锡市传染病医院

出　　处：《中国微循环》2007年第5期325-327,F0003,共4页Journal of Chinese Microcirculation

摘　　要：目的观察CD25/IL-2Ra在人肝毛细胆管上的表达,以利于肝毛细胆管分布、形态的观察。方法引用免疫组化方法观察CD25/IL-2Ra分子在人肝毛细胆管上的表达,并设立阳性对照(扁桃体)和CD4、CD8之间的片间对照。通过免疫荧光法观察CD25/IL-2Ra在肝毛细胆管上的表达,结果与免疫组化相一致。通过阻断试验进一步确认肝毛细胆管上CD25/IL-2Ra分子表达的特异性。通过与国际公认的CEA(多克隆抗体)作对照,进一步确认CD25/IL-2Ra是一种优良的肝毛细胆管标记物。结果常规免疫组化方法DAB显色CD25/IL-2Ra标记的毛细胆管显棕黄色条索状结构,显示结果比国际公认的CEA更佳,免疫荧光呈索状绿色荧光表达,阻断试验进一步确认肝毛细胆管上CD25/IL-2Ra分子的存在。结论免疫组化、免疫荧光法检测CD25/IL-2Ra表达来观察肝脏毛细胆管的分布、形态等,具有明确的特异性。Objective To observe the expressions of CD25/IL-2Ra on eholangiole of human liver for observing the pathology of the eholangiole of human liver. Methods We observed the expressions of CD25/IL-2Ra on eholangiole of liver by immunohistochemisty stain, at the same time set up positive contrast （ Tonsil tissue） and contrast of CD4, CD8. We observed the expressions of CD25/IL-2Ra on eholangiole of liver by immuno fluorescence, results showed no difference. We can further confirm the specificity of the expressions of CD25/IL-2Ra on eholangiole of human liver by blocking test. We made a contrasting test of CEA （ multi-clone immune body ） which is generally acknowledged by the medical faculty and further confirm that CD25/IL-2Ra is a fine sign of eholangiole of human liver. Results The eholangiole signed by CD25/IL-2Ra showed yellowishbrown strip funicular structure and it＇ s effect is better than CEA＇ s . The expression of blue fluorescence is shown by immunofluoreseenee. The blocking test further confirms the existence of CD25/IL-2Ra on eholangiole of human liver. Conclusion Nowadays the pathologists make CEA the sign of eholangiole of human liver. We investigate the expressions of CD25/IL-2Ra by immunohistochemisty and immunofluoreseenee and observe the distribution and morphology of eholangiole of human liver.

关 键 词：CD25/IL-2Ra肝毛细胆管 免疫组织化学染色 免疫荧光 阻断试验 

分 类 号：Q25[生物学—细胞生物学]