LC-MS/MS-MRM筛选血液中22种有毒生物碱成分  

Simultaneous Screening for 22 Poisonous Alkaloids in Blood by Liquid Chromatography-Tandem Mass Spectrometry with Multiple-Reaction Monitoring

作　　者：刘伟[1] 沈敏[2] 沈保华[2] 向平[2] 吴何坚[2] 

机构地区：[1]复旦大学上海医学院法医学系,上海200032 [2]司法部司法鉴定科学技术研究所,上海200063

出　　处：《法医学杂志》2007年第5期349-352,共4页Journal of Forensic Medicine

摘　　要：目的建立液相色谱-串联质谱法对血液中的22种常见有毒生物碱成分进行筛选分析。方法血液以丁丙诺菲为内标经液液提取后,用液相色谱-串联质谱仪以电喷雾电离(ESI+)、多反应监测(MRM)方式进行分析。结果以化合物的保留时间、两对母离子/子离子对定性,最低检出限为0.1～20ng/mL。结论该方法选择性佳、灵敏度高,适用于法医毒物分析和临床毒物分析中有毒生物碱的分析。Objective To establish a liquid chromatography-tandem mass chromatography （LC-MS/MS） method for the simultaneous screening for 22 poisonous alkaloids in blood. Methods This method involves a liquid-liquid extraction （LLE） followed by liquid chromatography-tandem mass spectrometry with multi- ple-reaction monitoring（MRM）. After blood was extracted with buprenorphine as the internal standard, the target compounds were analyzed with LC-MS/MS-ESI in the positive ionization mode. Results Identifica- tion was based on the compound＇s retention time and two precursor-to-product ion transitions. The limits of detection ranged from 0.1ng/mL to 20 ng/mL in blood. Conclusion The method was sufficiently selective and sensitive to detect poisonous alkaloids and can be applied in forensic and clinical toxicology.

关 键 词：有毒生物碱 筛选 液相色谱-串联质谱法 多反应检测 

分 类 号：DF795.4[医药卫生—法医学]