细胞ERK信号通路对单纯疱疹病毒Ⅱ型增殖复制的影响  

Effects of the host cell ERK signal transduction pathway on replication of typeⅡherpes simplex virus(HSV-2)

作　　者：罗凌琪[1] 屠静[1] 冯海[1] 张浩[1] 彭宜红[1] 

机构地区：[1]北京大学医学部病原生物学系,100083

出　　处：《中华微生物学和免疫学杂志》2007年第9期779-783,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金(30470084)

摘　　要：目的以Raf-MEK-ERK通路关键激酶MEK1(MAPK/extracellular signal-regulated kinase- kinase-1)为研究重点,利用MEK1-siRNA(small interfering RNA for MEK1)阻断宿主Raf-MEK-ERK通路,阐述该通路对病毒复制的作用。方法用HSV-2感染预转染MEK1-siRNA的HEK 293细胞,应用观察细胞病变效应、病毒滴度以及病毒蛋白表达等研究方法,揭示细胞Raf-MEK-ERK通路对HSV-2增殖复制作用的影响。结果MEK1-siRNA在特异性阻断MEK1表达的同时,能明显抑制HSV-2的复制。结论体外实验表明,Raf-MEK-ERK通路在HSV-2增殖复制过程中具有十分重要的作用,MEK1-siRNA具有药物开发的潜能。Objective To assess the importance of Raf-MEK-ERK pathway and to provide foundation for developing therapeutic and preventive strategy for viral infectious diseases. Methods To examine the effect of Raf-MEK-ERK signaling pathway on the rephcation of HSV-2, double-stranded siRNA designed specifically for silencing MEK1 expression was transfected into HEK-293 cells by LipofectAMINE, followed by HSV-2 infection. Methods employed includes microscopic observation of cytopathic effect（CPE）, virus titration, Western blot, etc. Results The expression of MEK1 was suppressed by transfection of its specific siRNA, which was associated with the inhibition of HSV-2-induced activation of ERK1/2. Conclusion MEK1 and its associated ERK pathway play an essential role in the replication of HSV-2. Silencing expression of MEK1 and perturbing ERK signaling pathway exhibit an evident anti-HSV-2 effect, suggesting it as a potential target for a therapeutic drug.

关 键 词：单纯疱疹病毒Ⅱ型 细胞外信号调节激酶 RNA干扰 细胞病变效应 

分 类 号：R373[医药卫生—病原生物学]