人类不同年龄段腰椎间盘组织细胞凋亡及其调控蛋白Bcl-2和Bax的表达  

APOPTOSIS OF INTERVERTEBRAL DISC CELLS IN DIFFERENT AGES OF HUMAN BODY AND THE EXPRESSION OF Bcl-2,Bax PROTEIN

作　　者：季爱玉[1] 于河见 沈若武[3] 王守彪[3] 

机构地区：[1]青岛大学医学院附属医院骨科,山东青岛266003 [2]青岛市市北区医院骨科 [3]青岛大学医学院人体解剖学教研室

出　　处：《青岛大学医学院学报》2007年第5期394-396,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：目的 探讨细胞凋亡及其调控蛋白在人类不同年龄段椎间盘组织退变中的作用及其可能存在的基因调控机制。方法 对0～58岁不同年龄段正常人腰椎间盘髓核细胞的形态结构进行苏木精-伊红染色光镜观察、TUNEL细胞凋亡检测、Bcl -2和Bax蛋白免疫组织化学染色。结果从胚胎后期开始,椎间盘髓核细胞数量随年龄增长而逐渐减少,到老年阶段髓核细胞的数量已经很少（H=95.123,P〈0.05）。从胚胎后期到成年,TUNEL阳性细胞率随年龄增长而逐渐降低,并降到整个生命过程中的最低点;继之,TUNEL阳性细胞率又逐年升高（F=205.391,P〈0.05）。自胚胎后期开始,Bcl-2蛋白就开始有较高水平的表达,但呈现逐年下降的趋势（F=81.385,P〈0.05）。Bax蛋白在胚胎后期即呈现较高水平表达,随年龄增长其表达水平逐渐降低（F=102.134,P〈0.05）;到成年后,Bax蛋白表达水平又有所升高,但不明显。结论 在整个生命过程中,随年龄增长大量腰椎间盘髓核细胞发生凋亡,细胞数量明显减少。Bcl-2蛋白可能参与了椎间盘细胞凋亡的调节,但表达水平较低,不能阻止细胞凋亡的发生。Bax蛋白的表达可能参与了整个生命过程中椎间盘髓核细胞的凋亡。Objective To investigate apoptosis of intervertebral disc cells and gene regulation in different ages of human body. Methods The intervertebral disc cells in different ages of human body were examined by hematoxylin and eosin staining, TdT-mediated dUTP nick-labeling （TUNEL） and Bcl-2, Box protein immunohistochemical method. Results From the later stage of embryo, the number of nucleus pulposus cells reduced gradually with the age, and the cells became few in the old-age stage （H=95. 123,P〈0.05）. From the later stage of embryo to adulthood, the rate of TUNEL positive cells reduced gradually to the lowest in the whole life, then increased with the age （F=205. 391 ,P〈0.05）. A higher expression of Bcl-2 protein could be seen in the later stage of embryo, but reduced gradually with the age （F=81. 385,P〈0.05）. From the later stage of embryo to adults, the rate of Bax protein positive cells reduced gradually with the age （F= 102. 134, P〈0.05） ; then the rate of Bax protein positive cells increased a little, year by year, from adults to old-age. Conclusion It is through apoptosis that nucleus pulposus cells of intervertebral disc die, the number of cells decrease obviously. Apoptosis of nucleus pulposus cells might be Bax-dependent, and because of the lower expression, Bcl-2 can not obstruct the occurrence of apoptosis.

关 键 词：椎间盘 髓核细胞 细胞凋亡 蛋白质BCL-2 蛋白质BAX 

分 类 号：R681.5[医药卫生—骨科学]