RP-HPLC法同时测定赤丹丸中黄芩苷与丹参酮ⅡA的含量  

Determination of baicalin and tanshinone Ⅱ A in Chidan pills by RP-HPLC

作　　者：李玉灵[1] 赵铁[1] 徐英宏[1] 阮国虎[1] 菅凌燕[1] 

机构地区：[1]中国医科大学附属盛京医院药剂科,辽宁沈阳110003

出　　处：《沈阳药科大学学报》2007年第10期623-626,共4页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省自然科学基金资助项目(20042189)

摘　　要：目的建立同时测定赤丹丸中黄芩苷、丹参酮ⅡA含量的方法。方法采用RP-HPLC法。使用Agilent Zorbax Extend C18柱（4.6 mm×250 mm,5μm）,以甲醇-水（磷酸调pH=3）-乙腈（体积比30∶50∶20~55∶15∶30）为流动相进行梯度洗脱,流速为1.0 mL.min-1,检测波长为270 nm,柱温为25℃。结果黄芩苷质量浓度在15.80~126.40 mg.L-1内与峰面积呈良好的线性关系,r=0.9999（n=5）,平均加样回收率为99.0%,RSD为1.28%;丹参酮ⅡA质量浓度在5.04~80.56 mg.L-1内与峰面积呈良好的线性关系,r=0.999 2（n=5）,平均加样回收率为98.3%,RSD为1.12%。结论RP-HPLC法简便、灵敏度高,重复性好,可用于赤丹丸的质量控制。Objective To establish an RP-HPLC method for simultaneous determination of the contents of baicalin and tanshinone Ⅱ A in Chidan （ traditional Chinese medicines） pills. Methods The column of Agilent Zorbax Extend C18（4.6 mm × 250 mm, 5 μm） was used, the mobile phase was consisted of methanol, water adjusted to pH 3 with phosphoric acid and acetonitrile, gradient elution was performed; the detection wavelength was set at 270 nm and the column temperature was 25 ℃. Results The linear range of baicalin and tanshinone Ⅱ A were 15.80 - 126.40 mg· L^- 1 and 5.04 - 80.56 mg· L^- 1 respectively. The average recoveries of baicalin and tanshinone Ⅱ A were 99.0 % with RSD of 1.28 %, 98.3 % with RSD of 1.12 % （ n = 5）, respectively. Conclusions The method is simple, sensitive and good reproducibility. It can be used for quality control of Chidan pills.

关 键 词：赤丹丸 黄芩苷 丹参酮ⅡA 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]